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Testing Data
(Figure A. FITC conjugated Mouse anti Human CD11b and Alexa Fluor 647 conjugated Mouse IgG2a isotype control . Figure B. FITC conjugated Mouse anti Human CD11b and Alexa Fluor 647 conjugated Mouse anti Human CD16 used for the evaluation of CD16 expression on bovine monocytes by flow cytometry.Image caption:Gating strategy utilized for flow cytometric analysis )
Mouse anti-Human CD16 Monoclonal Antibody | anti-CD16 antibody
Mouse anti Human CD16:RPE
Gene Names
FCGR3A; CD16; FCGR3; FCGR3@
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
CD16; Monoclonal Antibody; Mouse anti Human CD16:RPE; CD16 antibody | KD1; anti-CD16 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
KD1
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Applicable Applications for anti-CD16 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS
Immunogen
A polyclonal population of NK cells.
Fusion Partners
Spleen cells from immunised mice were fused with cells of the P3U1 myeloma cell line.
Conjugation
RPE
Preparation and Storage
Prior to reconstitution store at +4 degree C.
After reconstitution store at +4 degree C.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
After reconstitution store at +4 degree C.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD11b and Alexa Fluor 647 conjugated Mouse IgG2a isotype control . Figure B. FITC conjugated Mouse anti Human CD11b and Alexa Fluor 647 conjugated Mouse anti Human CD16 used for the evaluation of CD16 expression on bovine monocytes by flow cytometry.Image caption:Gating strategy utilized for flow cytometric analysis )
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD11b and Alexa Fluor 647 conjugated Mouse IgG2a isotype control . Figure B. FITC conjugated Mouse anti Human CD11b and Alexa Fluor 647 conjugated Mouse anti Human CD16 used for the evaluation of CD16 expression on bovine monocytes by flow cytometry.Image caption:Gating strategy utilized for flow cytometric analysis )
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD11b and PE conjugated Mouse IgG2a isotype control . Figure B. FITC conjugated Mouse anti Human CD11b and PE conjugated Mouse anti Human CD16 (MBS226106). All experiments performed )
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD11b and PE conjugated Mouse IgG2a isotype control . Figure B. FITC conjugated Mouse anti Human CD11b and PE conjugated Mouse anti Human CD16 (MBS226106). All experiments performed )
Testing Data
(Published customer image:FITC conjugated Mouse anti Bovine CD16 antibody, clone KD1 used to identify monocyte populations in bovime blood samples by flow cytometry.Image caption:Changes in the monocyte subsets of PBMCs in Holstein a)
Testing Data
(Published customer image:FITC conjugated Mouse anti Bovine CD16 antibody, clone KD1 used to identify monocyte populations in bovime blood samples by flow cytometry.Image caption:Changes in the monocyte subsets of PBMCs in Holstein a)
Testing Data
(Published customer image:FITC conjugated Mouse anti Bovine CD16 antibody, clone KD1 used to identify monocyte populations in bovime blood samples by flow cytometry.Image caption:Changes in the monocyte subsets of PBMCs in Holstein a)
Testing Data
(Published customer image:FITC conjugated Mouse anti Bovine CD16 antibody, clone KD1 used to identify monocyte populations in bovime blood samples by flow cytometry.Image caption:Changes in the monocyte subsets of PBMCs in Holstein a)
Related Product Information for anti-CD16 antibody
Mouse anti Human CD16 antibody, clone KD1 recognizes human CD16, a 50-65kDa cell surface molecule, which is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages (Moretta et al. 1990), whereas CD16B is primarily expressed by granulocytes (Bonecchi et al. 1999). Clone KD1 recognizes both forms of CD16 and will therefore recognize all cell types expressing CD16.
Mouse anti Human CD16, clone KD1 can be used to identify CD16 in a range of species including bovine (Boysen et al. 2010) and ovine (Elhmouzi-Younes et al. 2010).
Mouse anti Human CD16, clone KD1 can be used to identify CD16 in a range of species including bovine (Boysen et al. 2010) and ovine (Elhmouzi-Younes et al. 2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
28,050 Da
NCBI Official Full Name
Low affinity immunoglobulin gamma Fc region receptor III
NCBI Official Symbol
FCGR3A
NCBI Official Synonym Symbols
CD16; FCGR3; FCGR3@
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III
Protein Family
UniProt Gene Name
FCGR3
UniProt Synonym Gene Names
FCGRIII; IgG Fc receptor III; FcRIII
UniProt Entry Name
FCGR3_BOVIN
Uniprot Description
Is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Also mediates antibody-dependent cellular toxicity ().
Research Articles on CD16
Similar Products
Product Notes
The CD16 fcgr3 (Catalog #AAA226106) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human CD16:RPE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD16 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS. Researchers should empirically determine the suitability of the CD16 fcgr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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