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Testing Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD16:Biotin(MBS210312))

Mouse CD16 Monoclonal Antibody | anti-CD16 antibody

MOUSE ANTI HUMAN CD16:Low Endotoxin

Gene Names
FCGR3B; CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
CD16; Monoclonal Antibody; MOUSE ANTI HUMAN CD16:Low Endotoxin; anti-CD16 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
LNK16
Form/Format
Low Endotoxin
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
233
Applicable Applications for anti-CD16 antibody
Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul human whole blood.
Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200
Preparation
Purified IgG prepared by ion exchange chromatography
Immunogen
Normal human peripheral blood granulocytes
Fusion Partners
Target Species
Human
Preparation and Storage
Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD16:Biotin(MBS210312))

Testing Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD16:Biotin(MBS210312))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD16: Low Endotoxin)

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD16: Low Endotoxin)

Testing Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD16 (MBS212047))

Testing Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD16 (MBS212047))

Testing Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD16:FITC (MBS210627))

Testing Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD16:FITC (MBS210627))

Testing Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD16:Alexa Fluor 647 (MBS212047A647))

Testing Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD16:Alexa Fluor 647 (MBS212047A647))
Related Product Information for anti-CD16 antibody
Mouse anti Human CD16 antibody, clone LNK16 recognizes CD16, a 50-65kDa cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Mouse anti Human CD16 antibody, clone LNK16 has been reported to block the binding of IgG to the CD16 molecule (Tamm and Schmidt 1996).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,216 Da
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor III-B isoform 2
NCBI Official Synonym Full Names
Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
NCBI Official Symbol
FCGR3B
NCBI Official Synonym Symbols
CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III-B; Fc-gamma receptor IIIb (CD 16); fc-gamma RIII-beta; fc-gamma RIIIb; igG Fc receptor III-1
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III-B
Protein Family
UniProt Gene Name
FCGR3B
UniProt Synonym Gene Names
CD16B; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb
UniProt Entry Name
FCG3B_HUMAN

NCBI Description

The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1. [provided by RefSeq, Aug 2012]

Uniprot Description

Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.

Research Articles on CD16

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Product Notes

The CD16 fcgr3b (Catalog #AAA211192) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD16 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul human whole blood. Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the CD16 fcgr3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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