Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse anti Human CD8 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Mouse anti-Human CD8 Monoclonal Antibody | anti-CD8 antibody
Mouse Anti Human CD8: Amethyst Orange
Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul human whole blood.
1% Bovine Serum Albumin
This product should be stored undiluted.
Storage in frost free freezers is not recommended.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
This product contains a precipitate we recommend microcentrifugation before use.
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse anti Human CD8 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse anti Human CD8 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD8 antibody, clone LT8 followed by Histar Detection system . Low power)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD8 antibody, clone LT8 followed by Histar Detection system . Low power)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD8 antibody, clone LT8 followed by Histar Detection System . Medium power)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD8 antibody, clone LT8 followed by Histar Detection System . Medium power)
Testing Data
(Mouse anti Human CD8 antibody, clone LT8 used for the evaluation of CD8? exptession on T lymphocytes from marmoset blood, spleen and lung by flow cytometry.Image caption:The percentage of the total leucocyte count for various cell types identified in naive marmosets (a) cells in naïve blood, (b) in naive spleens, and (c) in naive lungs. CD8 T cells are expressed as a percentage of CD3+ cells. Bar represents the median value.From: M. Nelson and M. Loveday, “Exploring the Innate Immunological Response of an Alternative Nonhuman Primate Model of Infectious Disease; the Common Marmoset,”Journal of Immunology Research, vol. 2014, Article ID 913632, 8 pages, 2014.)
Testing Data
(Mouse anti Human CD8 antibody, clone LT8 used for the evaluation of CD8? exptession on T lymphocytes from marmoset blood, spleen and lung by flow cytometry.Image caption:The percentage of the total leucocyte count for various cell types identified in naive marmosets (a) cells in naïve blood, (b) in naive spleens, and (c) in naive lungs. CD8 T cells are expressed as a percentage of CD3+ cells. Bar represents the median value.From: M. Nelson and M. Loveday, “Exploring the Innate Immunological Response of an Alternative Nonhuman Primate Model of Infectious Disease; the Common Marmoset,”Journal of Immunology Research, vol. 2014, Article ID 913632, 8 pages, 2014.)
Testing Data
(Figure A. FITC conjugated mouse anti human CD3 and APC conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD3 and APC conjugated mouse anti human CD8 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock )
Testing Data
(Figure A. FITC conjugated mouse anti human CD3 and APC conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD3 and APC conjugated mouse anti human CD8 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock )
Testing Data
(Alexafluor 700 conjugated Mouse anti Human CD8 antibody, clone LT8 used for the evaluation of CD8 expression on PBMC by flow cytometry.Image caption:Gating stategy for cell surface marker analysis in uncultured and cultured PBMC. Gates were applied in the numerical order shown.From: Manivannan K, Rowan AG, Tanaka Y, Taylor GP, Bangham CRM (2016)CADM1/TSLC1 Identifies HTLV-1-Infected Cells and Determines Their Susceptibility to CTL-Mediated Lysis.PLoS Pathog 12(4): e1005560.)
Testing Data
(Alexafluor 700 conjugated Mouse anti Human CD8 antibody, clone LT8 used for the evaluation of CD8 expression on PBMC by flow cytometry.Image caption:Gating stategy for cell surface marker analysis in uncultured and cultured PBMC. Gates were applied in the numerical order shown.From: Manivannan K, Rowan AG, Tanaka Y, Taylor GP, Bangham CRM (2016)CADM1/TSLC1 Identifies HTLV-1-Infected Cells and Determines Their Susceptibility to CTL-Mediated Lysis.PLoS Pathog 12(4): e1005560.)
Testing Data
(Figure A. Alexa647 conjugated rat anti human CD3 and RPE conjugated Mouse IgG1 isotype control . Figure B. Alexa647 conjugated rat anti human CD3 and RPE conjugated mouse anti human CD8 . All experiments performed on red cell lysedand permeabilized Human peripheral blood gated on leukocytes in the presence of Human SeroBlock .)
Testing Data
(Figure A. Alexa647 conjugated rat anti human CD3 and RPE conjugated Mouse IgG1 isotype control . Figure B. Alexa647 conjugated rat anti human CD3 and RPE conjugated mouse anti human CD8 . All experiments performed on red cell lysedand permeabilized Human peripheral blood gated on leukocytes in the presence of Human SeroBlock .)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD8 antibody, clone LT8 followed by Histar Detection System . Medium power)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD8 antibody, clone LT8 followed by Histar Detection System . Medium power)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD8 antibody, clone LT8 followed by Histar Detection System . High power)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD8 antibody, clone LT8 followed by Histar Detection System . High power)
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse anti Human CD8 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse anti Human CD8 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells.
NCBI and Uniprot Product Information
NCBI Description
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. [provided by RefSeq, May 2020]
Research Articles on CD8
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Product Notes
The CD8 cd8a (Catalog #AAA225860) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD8: Amethyst Orange reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Min. Dilution: Neat, Max. Dilution: 1/10 Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul human whole blood. Researchers should empirically determine the suitability of the CD8 cd8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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