Western Blot (WB)
(Western Blot using anti-carcinoembryonic antigen antibody A5B7. human colorectal carcinoma tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of A5B7 at 0.01ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Mouse anti-Human carcinoembryonic Monoclonal Antibody
Anti-carcinoembryonic antigen [A5B7], Rabbit IgG, Kappa
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A affinity purified
Synonyms
carcinoembryonic; Monoclonal Antibody; Anti-carcinoembryonic antigen [A5B7]; Rabbit IgG; Kappa; CEA; CD66; Q7KZ28; Q13985; Q14081; anti-carcinoembryonic antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG, Kappa
Clone Number
A5B7
Specificity
The antibody is specific for human CEA.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-carcinoembryonic antibody
Radiolabelling, Therapeutic, Immunohistochemistry (IHC)
Application Notes
In IHC analysis, this CEA-specific antibody stains sections of colon tumour, with high levels of cell surface and cytoplasmic staining of malignant glands, and a more intense reaction in necrotic debris (Harwood et al, 1986). It does not cross-react with PMNs, erythrocytes or liver tissue. In an mouse model transplanted with the human colonic tumour MaWi, the weight of the xenograft has a profound effect on the uptake of a radiolabelled version of antibody (Pedley et al, 1987). In a xenograft tumour mouse model based on the adenocarcinoma cell line LS174T, both this antibody and its FAb have a significant effect on tumour growth when administered as radioimmunotherapeutic agents (Pedley et al, 1993). This antibody has also been shown to function as a radioimmunotherapy agent in a Phase I trial in gastrointestinal adenocarcinomas (Meyer et al, 2009), and has been shown in early clinical trials to target an enzyme capable of cleaving an inactive prodrug to release an active, cytotoxic drug to colorectal cancers (Bagshawe et al, 1991).
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised by immunising mice with both carcinoembryonic antigen (CEA) purified from the metastases of colonic tumours, and heat-treated CEA.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Western Blot (WB)
(Western Blot using anti-carcinoembryonic antigen antibody A5B7. human colorectal carcinoma tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of A5B7 at 0.01ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-carcinoembryonic antigen antibody A5B7. human colorectal carcinoma tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of A5B7 at 0.01ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Immunofluorescence (IF)
(Immunofluorescence staining of MCF7 cells using anti-carcinoembryonic antigen A5B7 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric mouse IgG version of A5B7 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of MCF7 cells using anti-carcinoembryonic antigen A5B7 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric mouse IgG version of A5B7 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-carcinoembryonic antibody
Describes the original generation of this antibody, and its characterisation in IHC analysis.
Recombinant monoclonal antibody to carcinoembryonic antigen. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma A5B7.
Recombinant monoclonal antibody to carcinoembryonic antigen. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma A5B7.
Product Categories/Family for anti-carcinoembryonic antibody
References
Harwood et al.; Br J Cancer. 1986 Jul;54(1):75-82. OriginPubPMID: 9329081
NCBI and Uniprot Product Information
UniProt Accession #
Protein Family
Similar Products
Product Notes
The carcinoembryonic (Catalog #AAA488522) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-carcinoembryonic antigen [A5B7], Rabbit IgG, Kappa reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's carcinoembryonic can be used in a range of immunoassay formats including, but not limited to, Radiolabelling, Therapeutic, Immunohistochemistry (IHC). In IHC analysis, this CEA-specific antibody stains sections of colon tumour, with high levels of cell surface and cytoplasmic staining of malignant glands, and a more intense reaction in necrotic debris (Harwood et al, 1986). It does not cross-react with PMNs, erythrocytes or liver tissue. In an mouse model transplanted with the human colonic tumour MaWi, the weight of the xenograft has a profound effect on the uptake of a radiolabelled version of antibody (Pedley et al, 1987). In a xenograft tumour mouse model based on the adenocarcinoma cell line LS174T, both this antibody and its FAb have a significant effect on tumour growth when administered as radioimmunotherapeutic agents (Pedley et al, 1993). This antibody has also been shown to function as a radioimmunotherapy agent in a Phase I trial in gastrointestinal adenocarcinomas (Meyer et al, 2009), and has been shown in early clinical trials to target an enzyme capable of cleaving an inactive prodrug to release an active, cytotoxic drug to colorectal cancers (Bagshawe et al, 1991). Researchers should empirically determine the suitability of the carcinoembryonic for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "carcinoembryonic, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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