Rabbit anti-Human LDB2 Polyclonal Antibody | anti-LDB2 antibody

LDB2, CT (LDB2, CLIM1, LIM domain-binding protein 2, Carboxyl-terminal LIM domain-binding protein 1, LIM domain-binding factor CLIM1) (FITC)

Cat. Num. AAA6315572

• Gene Names: LDB2; LDB1; CLIM1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LDB2; Polyclonal Antibody; CT (LDB2; CLIM1; LIM domain-binding protein 2; Carboxyl-terminal LIM domain-binding protein 1; LIM domain-binding factor CLIM1) (FITC); LIM domain-binding factor CLIM1; anti-LDB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-LDB2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: LDB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313-342 amino acids from the C-terminal region of human LDB2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(LDB2 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the LDB2 antibody detected the LDB2 protein (arrow).)

Immunohistochemistry (IHC)

(LDB2 Antibody (C-term) (AP14378b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LDB2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-LDB2 antibody

Genes encoding LIM domain-binding factors were initially isolated in a screen for proteins that physically interact with the LIM domains of nuclear proteins (summarized by Semina et al., 1998 [PubMed 9799849]). These proteins, such as the one encoded by the LDB2 gene, are capable of binding to a variety of transcription factors and are likely to function at enhancers to bring together diverse transcription factors and form higher order activation complexes or to block formation of such complexes (Jurata and Gill, 1997 [PubMed 9315627]). The family of genes encoding LIM domain-binding factors includes 2 members isolated from the mouse, Clim1 (Bach et al., 1997 [PubMed 9192866]) and Clim2/Lbd1/Nli (Agulnick et al., 1996 [PubMed 8918878]; Jurata et al., 1996 [PubMed 8876198]; Bach et al., 1997 [PubMed 9192866]) and their homologs cloned from the frog, chicken, and fly. The fact that LIM domain-binding factors are likely to be involved in the coordination of the transcriptional activity of many diverse factors might implicate them in human phenotypes characterized by multiple affected sites.

Product Categories/Family for anti-LDB2 antibody

Antibodies; Binding Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Hagg, S., et al. PLoS Genet. 5 (12), E1000754 (2009): Colland, F., et al. Genome Res. 14(7):1324-1332(2004). Kotaka, M., et al. J. Cell. Biochem. 78(4):558-565(2000). Bach, I., et al. Nat. Genet. 22(4):394-399(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 195546926
• NCBI GeneID: 9079
• NCBI Accession #: NP_001124306.1; NP_001281.1
• NCBI GenBank Nucleotide #: NM_001130834.1
• UniProt Accession #: O43679; O60619; O75480
• Molecular Weight: 42,793 Da
• NCBI Official Full Name: LIM domain-binding protein 2 isoform b
• NCBI Official Synonym Full Names: LIM domain binding 2
• NCBI Official Symbol: LDB2
• NCBI Official Synonym Symbols: LDB1; CLIM1
• NCBI Protein Information: LIM domain-binding protein 2; LDB-2; CLIM-1; LIM binding domain 2; LIM domain-binding factor-2; LIM domain-binding factor CLIM1; carboxyl-terminal LIM domain-binding protein 1
• UniProt Protein Name: LIM domain-binding protein 2
• Protein Family: LIM domain-binding protein
• UniProt Gene Name: LDB2
• UniProt Synonym Gene Names: CLIM1; LDB-2; CLIM-1
• UniProt Entry Name: LDB2_HUMAN

Uniprot Description

LDB2: Binds to the LIM domain of a wide variety of LIM domain- containing transcription factors. Belongs to the LDB family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: transcription factor complex; nucleus

Molecular Function: LIM domain binding; enzyme binding; transcription cofactor activity

Biological Process: hair follicle development; somatic stem cell maintenance; positive regulation of transcription from RNA polymerase II promoter