Immunofluorescence (IF)
(Immunofluorescence staining of human peripheral blood monocytes using anti-DCIR antibody at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Mouse anti-Human DCIR Monoclonal Antibody | anti-DCIR antibody
Anti-DCIR [9E8], Rabbit IgG, Kappa
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
DCIR; Monoclonal Antibody; Anti-DCIR [9E8]; Rabbit IgG; Kappa; CD367; Clec4A; C-type lectin domain family 4 member A; LLIR; CLECSF6; C-type lectin DDB27; C-type lectin superfamily member 6; Dendritic cell immunoreceptor; Lectin-like immunoreceptor; anti-DCIR antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG, Kappa
Clone Number
9E8
Specificity
The antibody is specific for human DCIR, which is expressed by monocytes, B cells, neutrophils, granulocytes and dendritic cells.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-DCIR antibody
Flow Cytometry (FC/FACS)
Application Notes
This antibody has been used in flow cytometric analysis of DCIR expression by peripheral blood mononuclear cells (Klechevsky et al, 2010). A recombinant humanised form of this antibody fused to protein antigens is under investigation as an antigen-targeting vaccine (Flamar et al, 2012), and has been shown to trigger antigen-specific CD8+ T cell immunity.
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised by immunising BALB/c mice with a dendritic cell immunoreceptor ectodomain-human Fc fusion protein.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of human peripheral blood monocytes using anti-DCIR antibody at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of human peripheral blood monocytes using anti-DCIR antibody at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-DCIR antibody 9E8 or the rabbit IgG1 version of 9E8 at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-DCIR antibody 9E8 or the rabbit IgG1 version of 9E8 at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-DCIR antibody
Describes the original generation of this antibody, and preliminary investigation into its use as an antigen-targeting vaccine.
Recombinant monoclonal antibody to DCIR. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 9E8.
Recombinant monoclonal antibody to DCIR. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 9E8.
Product Categories/Family for anti-DCIR antibody
References
Klechevsky et al.; Blood. 2010 Sep 9; 116(10): 1685-1697.
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The DCIR (Catalog #AAA488507) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-DCIR [9E8], Rabbit IgG, Kappa reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DCIR can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). This antibody has been used in flow cytometric analysis of DCIR expression by peripheral blood mononuclear cells (Klechevsky et al, 2010). A recombinant humanised form of this antibody fused to protein antigens is under investigation as an antigen-targeting vaccine (Flamar et al, 2012), and has been shown to trigger antigen-specific CD8+ T cell immunity. Researchers should empirically determine the suitability of the DCIR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DCIR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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