Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-TWEAK antibody MTW-1. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of MTW-1 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Rat anti-Mouse TWEAK Monoclonal Antibody | anti-TWEAK antibody
Anti-TWEAK [MTW-1], Rabbit IgG, Kappa
Gene Names
Tnfsf12; Dr3l; Apo3l; Dr3lg; Tweak
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
TWEAK; Monoclonal Antibody; Anti-TWEAK [MTW-1]; Rabbit IgG; Kappa; CD255; TNFSF12; TNF-related weak inducer of apoptosis; MTW1; MTW 1; Tumor necrosis factor ligand superfamily member 12; anti-TWEAK antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG, Kappa
Clone Number
MTW-1
Specificity
The antibody is specific for murine TWEAK. It has been shown not to react with TNF-alpha, FasL, and TRAIL.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TWEAK antibody
Blocking (BL), Flow Cytometry (FC/FACS)
Application Notes
The specificity of this antibody has been confirmed in flow cytometric analysis, where it reacts specifically with mTWEAK-transfected L5178Y cells, and not with parental L5178Y cells or cells transfected with related TNF family members (Nakayama et al, 2003). Additionally, this antibody has been used to assess TWEAK cell surface expression in flow cytometric analysis (Nakayama et al, 2003). This antibody has been shown to block CD8-mTWEAK cytotoxicity against L5178Y cells over-expressing murine Fn14, the TWEAK receptor (Nakayama et al, 2003). When administered to collagen-induced arthritis (CIA) mice, this blocking antibody has been shown to have anti-inflammatory and antiangiogenic effects, with associated improvements to both the clinical and histological manifestations of CIA (Kamata et al, 2006). Additionally, administration of this antibody to denervated mice attenuates denervation-induced skeletal muscle loss (Mittal et al, 2010). Finally, administration of this antibody to CD163-/- mice subject to hind limb ischaemia inhibits the increased perfusion resulting from CD163 deficiency (Akahori et al, 2015).
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised by immunising SD rats with CD8-mTWEAK, a fusion of the human CD8alpha extracellular region to murine TWEAK.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-TWEAK antibody MTW-1. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of MTW-1 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-TWEAK antibody MTW-1. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of MTW-1 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-TWEAK antibody
Describes the original generation of this antibody.
Recombinant monoclonal antibody to TWEAK. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MTW-1.
Recombinant monoclonal antibody to TWEAK. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MTW-1.
References
Nakayama et al.; Biochem Biophys Res Commun. 2003 Jul 11;306(4):819-25.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
27,441 Da
NCBI Official Full Name
Tumor necrosis factor ligand superfamily member 12
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 12
NCBI Official Symbol
Tnfsf12
NCBI Official Synonym Symbols
Dr3l; Apo3l; Dr3lg; Tweak
NCBI Protein Information
tumor necrosis factor ligand superfamily member 12; TNF-related weak inducer of apoptosis
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 12
UniProt Gene Name
Tnfsf12
UniProt Synonym Gene Names
TWEAK
UniProt Entry Name
TNF12_MOUSE
Uniprot Description
TNFSF12: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines (By similarity)
Protein type: Cytokine; Motility/polarity/chemotaxis; Membrane protein, integral; Apoptosis
Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane; extracellular region
Molecular Function: cytokine activity; tumor necrosis factor receptor binding
Biological Process: apoptosis; multicellular organismal development; positive regulation of protein catabolic process; immune response; angiogenesis; cell differentiation
Research Articles on TWEAK
Similar Products
Product Notes
The TWEAK tnfsf12 (Catalog #AAA488532) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-TWEAK [MTW-1], Rabbit IgG, Kappa reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TWEAK can be used in a range of immunoassay formats including, but not limited to, Blocking (BL), Flow Cytometry (FC/FACS). The specificity of this antibody has been confirmed in flow cytometric analysis, where it reacts specifically with mTWEAK-transfected L5178Y cells, and not with parental L5178Y cells or cells transfected with related TNF family members (Nakayama et al, 2003). Additionally, this antibody has been used to assess TWEAK cell surface expression in flow cytometric analysis (Nakayama et al, 2003). This antibody has been shown to block CD8-mTWEAK cytotoxicity against L5178Y cells over-expressing murine Fn14, the TWEAK receptor (Nakayama et al, 2003). When administered to collagen-induced arthritis (CIA) mice, this blocking antibody has been shown to have anti-inflammatory and antiangiogenic effects, with associated improvements to both the clinical and histological manifestations of CIA (Kamata et al, 2006). Additionally, administration of this antibody to denervated mice attenuates denervation-induced skeletal muscle loss (Mittal et al, 2010). Finally, administration of this antibody to CD163-/- mice subject to hind limb ischaemia inhibits the increased perfusion resulting from CD163 deficiency (Akahori et al, 2015). Researchers should empirically determine the suitability of the TWEAK tnfsf12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TWEAK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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