Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-5 antibody 2E3. Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 2E3 (blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
Mouse anti-Human IL-5 Monoclonal Antibody | anti-IL-5 antibody
Anti-IL-5 [2E3], Mouse IgG2a, Kappa
Reactivity
Human
Applications
Neutralization Assay, ELISA
Purity
Protein A affinity purified
Synonyms
IL-5; Monoclonal Antibody; Anti-IL-5 [2E3]; Mouse IgG2a; Kappa; interleukin 5; IL5; IL 5; Eosinophil differentiation factor; anti-IL-5 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a, Kappa
Clone Number
2E3
Specificity
The antibody is specific for human rIL-5, and does not cross-react with human rIL-1alpha, rIL-lbeta, rIL-4, rIL-8, rM-CSF, or rTGF-alpha.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IL-5 antibody
Neutralising, ELISA (EIA)
Application Notes
This antibody has been show to inhibit the binding of human rIL-5 to the alpha chain of the IL-5 receptor (Ames et al, 1995). This antibody inhibits the proliferation of murine B13 cells in response to human IL-5 (Ames et al, 1995).Neutralising antibodies against IL-5 represent a potential therapeutic strategy for the treatment of eosinophilia. The specificity of this antibody for human IL-5 has been confirmed in ELISA analysis (Ames et al, 1995).
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised by immunising CAF1 mice with human recombinant IL-5.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-5 antibody 2E3. Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 2E3 (blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-5 antibody 2E3. Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 2E3 (blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-IL-5 antibody
Describes the original generation of this antibody, and its characterisation and use in ELISA.
Recombinant monoclonal antibody to IL-5. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2E3.
Recombinant monoclonal antibody to IL-5. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2E3.
Product Categories/Family for anti-IL-5 antibody
References
Ames et al.; J. Immunol. 154 (12), 6355-6364 (1995). OriginPubPMID: 7759873
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The IL-5 (Catalog #AAA488517) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL-5 [2E3], Mouse IgG2a, Kappa reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-5 can be used in a range of immunoassay formats including, but not limited to, Neutralising, ELISA (EIA). This antibody has been show to inhibit the binding of human rIL-5 to the alpha chain of the IL-5 receptor (Ames et al, 1995). This antibody inhibits the proliferation of murine B13 cells in response to human IL-5 (Ames et al, 1995).Neutralising antibodies against IL-5 represent a potential therapeutic strategy for the treatment of eosinophilia. The specificity of this antibody for human IL-5 has been confirmed in ELISA analysis (Ames et al, 1995). Researchers should empirically determine the suitability of the IL-5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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