Testing Data
(Figure 1: Paraffin section of human cardiac muscle immunostained with 22D3 (1:500)
)
Mouse actin alpha-cardiac Monoclonal Antibody
Mouse anti actin alpha-cardiac
Reactivity
Caprine, Human, Rabbit, Rat, Swine, Zebrafish
Applications
ELISA, Immunohistochemistry, Western Blot
Synonyms
actin alpha-cardiac; Monoclonal Antibody; Mouse anti actin alpha-cardiac; anti-actin alpha-cardiac antibody
Host
Mouse
Reactivity
Caprine, Human, Rabbit, Rat, Swine, Zebrafish
Clonality
Monoclonal
Isotype
IgG1
Clone Number
22D3
Specificity
Clone 22D3 is an antibody highly specific for a- cardiac actin, and does not cross react with other actin isoforms.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-actin alpha-cardiac antibody
ELISA (EIA), Immunohistochemistry (IHC) (frozen), Western Blot (WB)
Application Notes
22D3 is useful for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and ELISA. Optimal antibody dilutions should be determined by titRation; recommended range is 1:100 - 1:1000 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting appliCations.
Source Note
22D3 is a Mouse monoclonal IgG1 antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of alpha-cardiac actin with an acetylated N-terminus (Ac-DDEETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
Reactivity Note
The epitope recognized by alpha-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Testing Data
(Figure 1: Paraffin section of human cardiac muscle immunostained with 22D3 (1:500) )
Testing Data
(Figure 1: Paraffin section of human cardiac muscle immunostained with 22D3 (1:500)
)
Testing Data
(Figure 2: Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo )
Testing Data
(Figure 2: Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo
)
Testing Data
(Figure 3: Immunofluorescence staining of developing myocardium in 1 month old zebrafish embryo )
Testing Data
(Figure 3: Immunofluorescence staining of developing myocardium in 1 month old zebrafish embryo
)
Related Product Information for anti-actin alpha-cardiac antibody
Among the six actin isoforms described in mammals, two are found in virtually all cells (beta- and gamma-cytoplasmic), two are detected in smooth muscle cells (alpha- and gamma-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (alpha-skeletal) and one in cardiac (alpha-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebRates. Cardiac alpha-actin, which is the main actin isoform in the adult heart, has also been shown to be the predominant form in early muscle development. In later development the expression of alpha-cardiac actin is down regulated and alpha-skeletal actin becomes the dominant isoform in the adult skeletal muscle.
Product Categories/Family for anti-actin alpha-cardiac antibody
References
Vandekerckhove, J. and Weber, K. (1978). At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4), 783-802.Vandekerckhove, J., Bugaisky, G. and Buckingham, M. (1986). Simultaneous expression of skeletal muscle and heart actin proteins in various striated muscle tissues and cells. J Biol Chem. 261(4),1838-43.Suurmeijer, A.J., Clément, S., Francesconi, A., Bocchi, L., Angelini, A., Van Veldhuisen, D.J., Spagnoli, L.G., Gabbiani, G. and Orlandi, A. (2003). Alpha-actin isoform distribution in normal and failing Human heart: a morphological, morphometric, and biochemical study. J Pathol. 199(3), 387-97.Chaponnier, C. and Gabbiani, G. (2004). Pathological situations characterized by altered actin isoform expression. J Pathol. 204(4), 386-95.Driesen, R.B., Verheyen F.K., Debie, W., Blaauw, E., Babiker, F.A., Cornelussen, R.N., Ausma, J., Lenders, M.H., Borgers, M., Chaponnier, C. and Ramaekers, F.C. (2009). Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies. J Cell Mol Med 13(5), 896-908.
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Product Notes
The actin alpha-cardiac (Catalog #AAA570264) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti actin alpha-cardiac reacts with Caprine, Human, Rabbit, Rat, Swine, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's actin alpha-cardiac can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) (frozen), Western Blot (WB). 22D3 is useful for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and ELISA. Optimal antibody dilutions should be determined by titRation; recommended range is 1:100 - 1:1000 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the actin alpha-cardiac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "actin alpha-cardiac, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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