Mouse Actin, pan Monoclonal Antibody | anti-Act5C antibody

Actin, pan

Cat. Num. AAA632115

• Gene Names: Act5C; A; Ac5C; act; Act; act 5C; Act-5C; Act5; act5C; Act5c; ACT5C; actin; Actin; Actin/BAP47; actin5C; Actin5C; anon-EST:fe2D2; Bap47; BAP47; beta-actin; beta-actin/Bap47; CG4027; cyt5C; DmelCG4027; l(1)G0009; l(1)G0010; l(1)G0025; l(1)G0079; l(1)G0117; l(1)G0
• Reactivity: Bovine, Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Actin; pan; Monoclonal Antibody; Anti -Actin; anti-Act5C antibody

• Host: Mouse
• Reactivity: Bovine, Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3F12
• Specificity: Recognizes all six known vertebrate isoforms of actin (MW~42kD) and two cytoplasmic actins (beta, gamma) which are highly homologous to one another but which differ from the muscle actins at about 25aa. A0760-51 is highly recommended for monitoring total protein load on Western blots. Species Crossreactivity: Human, bovine, mouse, canine, porcine, rabbit, rat, chicken, Dictyostelium discoideum, Physarum polycephalum.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide before the addition of glycerol to 40%. Also available without BSA and azide. See A0760-51X

• Applicable Applications for anti-Act5C antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry.; Dilution: Immunoprecipitation (Denatured verified) (Use Protein G): 2ug/mg protein lysate; Western Blot: 0.5ug/ml for 2hrs at RT; Immunohistochemistry (formalin/paraffin): 1:200 for 30 min; Requires boiling tissues sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20min.; Positive Control:; HeLa cells and skeletal muscle.
• Immunogen: Chicken gizzard actin. Cellular Localization: Cytoplasmic
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot of human cells using MBS632115.)

Western Blot (WB)

(Immunoprecipitation/Western Blot of human cells using MBS632115.)

Related Product Information for anti-Act5C antibody

Actin, an ubiquitous protein in eukaryotes, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle beta- and gamma-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. Alpha-cardiac and alpha-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, alpha- and gamma-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate contractile potentials for muscle cells. Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin. The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments. It has been reported that actin is hyperphosphorylated in primary breast tumors. Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle. Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis.

Product Categories/Family for anti-Act5C antibody

Antibodies; Abs to Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 62473526
• NCBI GeneID: 31521
• NCBI Accession #: NP_001014726.1
• NCBI GenBank Nucleotide #: NM_001014726.2
• UniProt Accession #: P10987; Q24227; Q6YN46; Q9U5X7; Q9W460; A4V404
• Molecular Weight: 41,822 Da
• NCBI Official Full Name: actin 5C, isoform C
• NCBI Official Synonym Full Names: Actin 5C
• NCBI Official Symbol: Act5C
• NCBI Official Synonym Symbols: A; Ac5C; act; Act; act 5C; Act-5C; Act5; act5C; Act5c; ACT5C; actin; Actin; Actin/BAP47; actin5C; Actin5C; anon-EST:fe2D2; Bap47; BAP47; beta-actin; beta-actin/Bap47; CG4027; cyt5C; DmelCG4027; l(1)G0009; l(1)G0010; l(1)G0025; l(1)G0079; l(1)G0117; l(1)G0
• NCBI Protein Information: CG4027 gene product from transcript CG4027-RC; 5C actin; Act5C-PA; Act5C-PB; Act5C-PC; Act5C-PD; CG4027-PA; CG4027-PB; CG4027-PC; CG4027-PD; Dm-actin5C; F-actin; G-actin; actin 5 C; actin 5C; actin 5c; actin A1; anon-fast-evolving-2D2; cellular cytoskeletal beta-actin
• UniProt Protein Name: Actin-5C
• Protein Family: Actin
• UniProt Gene Name: Act5C
• UniProt Entry Name: ACT1_DROME

Uniprot Description

Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.

Subcellular location: Cytoplasm › cytoskeleton.

Post-translational modification: Oxidation of Met-45 by Mical to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.

Miscellaneous: In Drosophila there are 6 closely related actin genes.

Sequence similarities: Belongs to the actin family.