Mouse actin alpha-skeletal Monoclonal Antibody | anti-ACTA1 antibody

Mouse anti actin alpha-skeletal

Cat. Num. AAA570258

• Gene Names: ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
• Reactivity: Caprine, Human, Rabbit, Rat, Swine.
• Applications: ELISA, Immunoblot, Western Blot, Immunohistochemistry, Immunohistochemistry, Western Blot
• Synonyms: actin alpha-skeletal; Monoclonal Antibody; Mouse anti actin alpha-skeletal; anti-ACTA1 antibody

• Host: Mouse
• Reactivity: Caprine, Human, Rabbit, Rat, Swine.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3B3
• Specificity: Clone 3B3 is an antibody highly specific for alpha-skeletal actin, and does not cross react with other actin isoforms.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-ACTA1 antibody: ELISA, Immunoblotting, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blotting.
• Application Notes: 3B3 is useful for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and ELISA. Optimal antibody dilutions should be determined by titRation; recommended range is 1:100 - 1:1000 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting appliCations.
• Source Note: 3B3 is a Mouse monoclonal IgG1 antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of alpha-skeletal actin with an acetylated N-terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
• Reactivity Note: The epitope recognized by alpha-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.
• Product Type: Primary Antibody
• Preparation and Storage: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Testing Data

(Paraffin sections of human striated skeletal muscle (above) and striated muscle (below) from human tongue immunostained with 3B3 (1:250))

Testing Data

Related Product Information for anti-ACTA1 antibody

Among the six actin isoforms described in mammals, two are found in virtually all cells (beta- and gamma-cytoplasmic), two are detected in smooth muscle cells (alpha- and gamma-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (alpha-skeletal) and one in cardiac (alpha-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebRates. Skeletal alpha-actin may also be expressed in the mammalian heart. Although during the late stage of fetal development alpha-cardiac actin becomes the main isoform in the myocardium, alpha-skeletal actin might still make up for almost half of its total striated muscle actin content.

Product Categories/Family for anti-ACTA1 antibody

Cytoskeletal; Cardiovascular; Cell differentiation; Cytoskeleton; Stem cell research

References

Vandekerckhove, J. and Weber, K. (1978). At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4), 783-802.Vandekerckhove, J., Bugaisky, G. and Buckingham, M. (1986). Simultaneous expression of skeletal muscle and heart actin proteins in various striated muscle tissues and cells. J Biol Chem. 261(4),1838-43.Suurmeijer, A.J., Clément, S., Francesconi, A., Bocchi, L., Angelini, A., Van Veldhuisen, D.J., Spagnoli, L.G., Gabbiani, G. and Orlandi, A. (2003). Alpha-actin isoform distribution in normal and failing Human heart: a morphological, morphometric, and biochemical study. J Pathol. 199(3), 387-97.Chaponnier, C. and Gabbiani, G. (2004). Pathological situations characterized by altered actin isoform expression. J Pathol. 204(4), 386-95.Driesen, R.B., Verheyen F.K., Debie, W., Blaauw, E., Babiker, F.A., Cornelussen, R.N., Ausma, J., Lenders, M.H., Borgers, M., Chaponnier, C. and Ramaekers, F.C. (2009). Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies. J Cell Mol Med 13(5), 896-908.

NCBI and Uniprot Product Information

• NCBI GI #: 4501881
• NCBI GeneID: 58
• NCBI Accession #: NP_001091.1
• NCBI GenBank Nucleotide #: NM_001100.3
• UniProt Accession #: P68133; P02568; P99020; Q5T8M9
• NCBI Official Full Name: actin, alpha skeletal muscle
• NCBI Official Synonym Full Names: actin, alpha 1, skeletal muscle
• NCBI Official Symbol: ACTA1
• NCBI Official Synonym Symbols: ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
• NCBI Protein Information: actin, alpha skeletal muscle; nemaline myopathy type 3
• UniProt Protein Name: Actin, alpha skeletal muscle
• UniProt Gene Name: ACTA1
• UniProt Synonym Gene Names: ACTA
• UniProt Entry Name: ACTS_HUMAN

NCBI Description

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]

Uniprot Description

ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family.

Protein type: Cell development/differentiation; Contractile; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: extracellular space; sarcomere; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeleton

Molecular Function: protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP binding

Biological Process: skeletal muscle fiber adaptation; muscle contraction; response to steroid hormone stimulus; response to mechanical stimulus; muscle thin filament assembly; response to lithium ion; response to extracellular stimulus; skeletal muscle fiber development; cell growth; muscle filament sliding

Disease: Nemaline Myopathy 3; Myopathy, Congenital, With Fiber-type Disproportion