Mouse Actin, alpha, Smooth Muscle Monoclonal Antibody | anti-ACTA2 antibody

Actin, alpha, Smooth Muscle (BSA, Glycerol & Azide Free)

Cat. Num. AAA631457

• Gene Names: ACTA2; AAT6; ACTSA; MYMY5
• Reactivity: Bovine, Chicken, Human, Mouse, Rabbit, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Actin; alpha; Smooth Muscle; Monoclonal Antibody; Smooth Muscle (BSA; Glycerol & Azide Free); Anti -Actin; anti-ACTA2 antibody

• Host: Mouse
• Reactivity: Bovine, Chicken, Human, Mouse, Rabbit, Rat
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 0.N.5
• Specificity: Recognizes the alpha-smooth muscle isoform of actin. Stains smooth muscle cells in vessel walls, gut walls, and myometrium. Myoepithelial cells in breat and salivary gland are also stained. Reacts with tumors arising from smooth muscles and myoepithelial cells. Does not crossreact with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial). Species Crossreactivity: Human, baboon, bovine, rabbit, mouse, rat and chicken.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4. No preservatives added. No stabilizing proteins added. Glycerol free. Also available with BSA and azide.

• Applicable Applications for anti-ACTA2 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Immunohistochemistry.; Dilution: Immunohistochemistry (Frozen & formalin/paraffin): 0.5-1ug/ml for 30min at RT. No special pretreatment is required for the Immunohistochemical staining of formalin/paraffin tissues.
• Immunogen: N-terminal decapeptide of the alpha smooth muscle isoform of actin; acetylated at the N-terminus. Epitope Location: Acetyl group and the first 4 amino acids on the N-terminal end of the peptidic chain of alpha-smooth actin. Cellular Localization: Cytoplasmic.
• Positive Control: Smooth muscle or leiomyosarcoma
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemistry of formalin-fixed, paraffin-embedded human leiomyoma using A0760-26 followed by peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of smooth muscle cells.)

Related Product Information for anti-ACTA2 antibody

Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.

Product Categories/Family for anti-ACTA2 antibody

Antibodies; Abs to Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 119570540
• NCBI GeneID: 59
• UniProt Accession #: P62736; P03996; P04108; Q6FI19; B2R8A4
• Molecular Weight: 42,009 Da
• NCBI Official Full Name: actin, alpha 2, smooth muscle, aorta, isoform CRA_a
• NCBI Official Synonym Full Names: actin, alpha 2, smooth muscle, aorta
• NCBI Official Symbol: ACTA2
• NCBI Official Synonym Symbols: AAT6; ACTSA; MYMY5
• NCBI Protein Information: actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein
• UniProt Protein Name: Actin, aortic smooth muscle
• Protein Family: Actin
• UniProt Gene Name: ACTA2
• UniProt Synonym Gene Names: ACTSA; ACTVS
• UniProt Entry Name: ACTA_HUMAN

NCBI Description

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

Uniprot Description

ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.

Protein type: Cytoskeletal; Contractile; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: extracellular space; smooth muscle contractile fiber; protein complex; cytoplasm; cytosol; actin cytoskeleton

Molecular Function: protein kinase binding; ATP binding

Biological Process: muscle contraction; regulation of blood pressure; response to virus; vascular smooth muscle contraction

Disease: Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome; Aortic Aneurysm, Familial Thoracic 6