Western Blot (WB)
(Western blot (WB) analysis of Alpha-actin polyclonal antibody at 1:500 dilutionLaneA: C6 whole cell lysateLaneB: AML-12 whole cell lysate)
Rabbit Alpha-actin Polyclonal Antibody | anti-ACTC1 antibody
Alpha-actin polyclonal antibody
Gene Names
ACTC1; ACTC; ASD5; CMD1R; CMH11; LVNC4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Synonyms
Alpha-actin; Polyclonal Antibody; Alpha-actin polyclonal antibody; Actin; alpha cardiac muscle 1; Alpha-cardiac actin; intermediate form; ACTC1; ACTC; anti-ACTC1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Alpha-actin polyclonal antibody detects endogenous levels of Alpha-actin protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ACTC1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:200
IHC: 1:50~1:200
Immunogen
Synthetic peptide, corresponding to Human Alpha-actin
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.
Western Blot (WB)
(Western blot (WB) analysis of Alpha-actin polyclonal antibody at 1:500 dilutionLaneA: C6 whole cell lysateLaneB: AML-12 whole cell lysate)
Western Blot (WB)
(Western blot (WB) analysis of Alpha-actin polyclonal antibody at 1:500 dilutionLaneA: C6 whole cell lysateLaneB: AML-12 whole cell lysate)
Related Product Information for anti-ACTC1 antibody
Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle beta- and gamma-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. alpha-cardiac and alpha-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, alpha- and gamma-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells. Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin. The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments. Research studies have shown that actin is hyperphosphorylated in primary breast tumors. Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies. Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
377
NCBI Official Full Name
actin, alpha cardiac muscle 1 proprotein
NCBI Official Synonym Full Names
actin, alpha, cardiac muscle 1
NCBI Official Symbol
ACTC1
NCBI Official Synonym Symbols
ACTC; ASD5; CMD1R; CMH11; LVNC4
NCBI Protein Information
actin, alpha cardiac muscle 1
UniProt Protein Name
Actin, alpha cardiac muscle 1
Protein Family
UniProt Gene Name
ACTC1
UniProt Synonym Gene Names
ACTC
UniProt Entry Name
ACTC_HUMAN
Similar Products
Product Notes
The ACTC1 actc1 (Catalog #AAA3014939) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Alpha-actin polyclonal antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha-actin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500~1:1000 IHC: 1:50~1:200. Researchers should empirically determine the suitability of the ACTC1 actc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-actin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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