Testing Data
(Figure 1: Methanol fixed human dermal fibroblast immunostained with 4C2 (1:500)
)
Mouse actin beta-cytoplasmic Monoclonal Antibody
Mouse anti actin beta-cytoplasmic
Reactivity
Chicken, Human, Mouse, Rabbit, Rat, Swine, Zebrafish
Applications
Immunohistochemistry, Immunohistochemistry, Western Blot
Synonyms
actin beta-cytoplasmic; Monoclonal Antibody; Mouse anti actin beta-cytoplasmic; anti-actin beta-cytoplasmic antibody
Host
Mouse
Reactivity
Chicken, Human, Mouse, Rabbit, Rat, Swine, Zebrafish
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4C2
Specificity
4C2 is a Mouse monoclonal Ig1 antibody highly specific for beta-cytoplasmic actin.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-actin beta-cytoplasmic antibody
Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
Application Notes
4C2 is useful for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting, flow cytometry and ELISA. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:500 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting appliCations.
Source Note
4C2 is a Mouse monoclonal IgG1 antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of beta-cytoplasmic actin with an acetylated N-terminus (Ac-DDDIAALVV-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue. The antibody does not cross react with other actin isoforms.
Reactivity Note
The epitope recognized by alpha-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Testing Data
(Figure 1: Methanol fixed human dermal fibroblast immunostained with 4C2 (1:500) )
Testing Data
(Figure 1: Methanol fixed human dermal fibroblast immunostained with 4C2 (1:500)
)
Testing Data
(Figure 2: Immunofluorescence staining of 3 days old zebrafish embryo )
Testing Data
(Figure 2: Immunofluorescence staining of 3 days old zebrafish embryo
)
Testing Data
(Figure 3: Immunofluorescence staining of 1 month old zebrafish embryo )
Testing Data
(Figure 3: Immunofluorescence staining of 1 month old zebrafish embryo
)
Related Product Information for anti-actin beta-cytoplasmic antibody
Among the six actin isoforms described in mammals, two are found in virtually all cells (beta- and gamma-cytoplasmic), two are detected in smooth muscle cells (alpha- and gamma-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (alpha-skeletal) and one in cardiac (alpha-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebRates. beta- and gamma-cytoplasmic actin play crucial roles during various key cellular processes. Whereas beta-actin is preferentially localized in stress fibers, circular bundles and at cell-cell contacts, suggesting a role in cell attachment and contraction, gamma-actin displays a more versatile organization, according to cell activities. In moving cells, gamma-actin is mainly organized as a meshwork in cortical and lamellipodial structures, suggesting a role in cell motility. beta- and gamma-actin depleted fibroblasts exhibit distinct changes in motility compared with their controls, suggesting a specific role for each isoform in cell locomotion.
Product Categories/Family for anti-actin beta-cytoplasmic antibody
References
Vandekerckhove, J. and Weber, K. (1978). At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4), 783-802.Bassell, G.J., Zhang, H., Byrd, A.L., Femino, A.M., Singer, R.H., Taneja, K.L., Lifshitz, L.M., Herman, I.M. and Kosik, K.S. (1998). Sorting of beta-actin mRNA and protein to neurites and growth cones in culture. J Neurosci. 1998 18(1), 251-65.Dugina, V., Zwaenepoel, I., Gabbiani, G., Clément, S. and Chaponnier, C. (2009). beta- and gamma-cytoplasmic actins display distinct distribution and functional diversity. J Cell Sci 122, 2980-88.Gallant, C., Appel, S., Graceffa, P., Leavis, P., Lin, J.J., Gunning, P.W., Schevzov, G., Chaponnier, C., Degnore, J., Lehman, W. and Morgan, K.G. (2011). Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells. Am J Physiol Cell Physiol. 300(6), 1356-65.
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Product Notes
The actin beta-cytoplasmic (Catalog #AAA570241) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti actin beta-cytoplasmic reacts with Chicken, Human, Mouse, Rabbit, Rat, Swine, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's actin beta-cytoplasmic can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB). 4C2 is useful for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting, flow cytometry and ELISA. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:500 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the actin beta-cytoplasmic for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "actin beta-cytoplasmic, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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