SDS-PAGE
LMTK3 recombinant protein
LMTK3 recombinant protein
Gene Names
Lmtk3; aatyk3; BC059845
Applications
Western Blot
Synonyms
LMTK3; LMTK3 recombinant protein; AATYK3; MGC90743
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
63-484
Sequence Length
4846
Applicable Applications for LMTK3 recombinant protein
Western Blot (WB)
Type
Recombinant Fusion Protein
Species
Mouse
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant mouse LMTK3 (63-484) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for LMTK3 recombinant protein
Recombinant mouse LMTK3 (63-484) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: LMTK3, or Lemur tyrosine kinase 3, belongs to the LMR subfamily of tyr protein kinases. LMTK3 decreases the activity of protein kinase C (PKC) and also leads to phosphorylation of AKT (Ser473), thereby increasing binding of forkhead box O3 (FOXO3) to the ESR1 promoter. LMTK3 phosphorylates ERα, protecting it from proteasomal degradation in vitro. Knockdown of LMTK3 leads to a reduction of tumor volume in an orthotopic mouse model and abrogates proliferation of ERα+ but not ERα- cells, indicative of its role in ERα activity. These findings reveal LMTK3 as a new therapeutic target for breast cancer (1).
Scientific Background: LMTK3, or Lemur tyrosine kinase 3, belongs to the LMR subfamily of tyr protein kinases. LMTK3 decreases the activity of protein kinase C (PKC) and also leads to phosphorylation of AKT (Ser473), thereby increasing binding of forkhead box O3 (FOXO3) to the ESR1 promoter. LMTK3 phosphorylates ERα, protecting it from proteasomal degradation in vitro. Knockdown of LMTK3 leads to a reduction of tumor volume in an orthotopic mouse model and abrogates proliferation of ERα+ but not ERα- cells, indicative of its role in ERα activity. These findings reveal LMTK3 as a new therapeutic target for breast cancer (1).
Product Categories/Family for LMTK3 recombinant protein
References
1. Giamas G et al: Kinome screening for regulators of the estrogen receptor identifies LMTK3 as a new therapeutic target in breast cancer. Nat Med. 2011 Jun;17(6):715-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~75 kDa
NCBI Official Full Name
Mus musculus lemur tyrosine kinase 3 (Lmtk3), mRNA
NCBI Official Synonym Full Names
lemur tyrosine kinase 3
NCBI Official Symbol
Lmtk3
NCBI Official Synonym Symbols
aatyk3; BC059845
NCBI Protein Information
serine/threonine-protein kinase LMTK3; apoptosis-associated tyrosine kinase 3
UniProt Protein Name
Serine/threonine-protein kinase LMTK3
Protein Family
UniProt Gene Name
Lmtk3
UniProt Synonym Gene Names
Aatyk3
UniProt Entry Name
LMTK3_MOUSE
Uniprot Description
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Subcellular location: Membrane; Single-pass membrane protein
Probable. Cell projection › axon. Cell projection › dendrite. Note: Punctate pattern in cell projections. Ref.1
Tissue specificity: Expressed in brain. Predominantly expressed in the cerebellum and hippocampal formation (at protein level). Ref.1
Developmental stage: Up-regulated during postnatal development, and expressed in adult stage. Ref.1
Post-translational modification: Autophosphorylated. Ref.1
Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family.Contains 1 protein kinase domain.
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Product Notes
The LMTK3 lmtk3 (Catalog #AAA515197) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 63-484 with tag GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant mouse LMTK3 (63-484) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. AAA Biotech's LMTK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the LMTK3 lmtk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LMTK3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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