SDS-PAGE
ULK4 recombinant protein
ULK4 recombinant protein
Gene Names
ULK4; FAM7C1; REC01035
Applications
Western Blot
Synonyms
ULK4; ULK4 recombinant protein; DKFZp434E1822; FAM7C1; FLJ20574; REC01035
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
1-616
Sequence Length
4425
Applicable Applications for ULK4 recombinant protein
Western Blot (WB)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant human ULK4 (1-616) was expressed by baculovirus in Sf9 insect cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for ULK4 recombinant protein
Recombinant human ULK4 (1-616) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: ULK4, or Unc-51-like kinase 4, is a Ser/Thr protein kinase that is a member of the APG1/unc-51/ULK1 subfamily. ULK4 contains five HEAT repeats and a catalytic kinase domain. The ULK4 gene is conserved in many species including chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, A.thaliana, and rice (1). ULK4 has been implicated as a gene involved in the development of hydrocephalus (2). This neurologic disorder in animal models is being used to elucidate factors responsible for the excessive accumulation of cerebrospinal fluid in hydrocephalic humans.
Scientific Background: ULK4, or Unc-51-like kinase 4, is a Ser/Thr protein kinase that is a member of the APG1/unc-51/ULK1 subfamily. ULK4 contains five HEAT repeats and a catalytic kinase domain. The ULK4 gene is conserved in many species including chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, A.thaliana, and rice (1). ULK4 has been implicated as a gene involved in the development of hydrocephalus (2). This neurologic disorder in animal models is being used to elucidate factors responsible for the excessive accumulation of cerebrospinal fluid in hydrocephalic humans.
Product Categories/Family for ULK4 recombinant protein
References
1. Krupnova T et al: Microtubule-associated kinase-like protein RUNKEL needed [corrected] for cell plate expansion in Arabidopsis cytokinesis. Curr Biol. 2009 Mar 24;19(6):518-23.
2. Vogel, P. et al: Congenital Hydrocephalus in Genetically Engineered Mice. Vet Pathol. 2011 Jul 11.
2. Vogel, P. et al: Congenital Hydrocephalus in Genetically Engineered Mice. Vet Pathol. 2011 Jul 11.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~97 kDa
NCBI Official Full Name
Homo sapiens unc-51 like kinase 4 (ULK4), mRNA
NCBI Official Synonym Full Names
unc-51 like kinase 4
NCBI Official Symbol
ULK4
NCBI Official Synonym Symbols
FAM7C1; REC01035
NCBI Protein Information
serine/threonine-protein kinase ULK4; unc-51-like kinase 4
UniProt Protein Name
Serine/threonine-protein kinase ULK4
Protein Family
UniProt Gene Name
ULK4
UniProt Entry Name
ULK4_HUMAN
Uniprot Description
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Domain: The protein kinase domain is predicted to be catalytically inactive.
Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.Contains 5 HEAT repeats.Contains 1 protein kinase domain.
Sequence caution: The sequence BAA91270.1 differs from that shown. Reason: Frameshift at position 489.
Research Articles on ULK4
Similar Products
Product Notes
The ULK4 ulk4 (Catalog #AAA515119) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-616 with tag GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant human ULK4 (1-616) was expressed by baculovirus in Sf9 insect cells. AAA Biotech's ULK4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the ULK4 ulk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ULK4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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