SDS-PAGE

MO25 alpha recombinant protein

Gene Names

CAB39; MO25; CGI-66

Applications

Western Blot

Synonyms

MO25 alpha; MO25 alpha recombinant protein; CAB39; CGI-66; FLJ22682; MO25alpha Protein; MO25a Protein

Ordering
Cat. Num. AAA515254 For Research Use Only!

Host

Sf9 insect cells

Form/Format

50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Sequence Length

3846

Applicable Applications for MO25 alpha recombinant protein

Kinase Assay, Western Blot (WB)

Type

Recombinant Fusion Protein

Species

Human

Tag Information

GST tag

Expression System

Sf9 insect cells using baculovirus

Source Note

Recombinant full-length human MO25alpha was expressed by baculovirus in Sf9 insect cells

Preparation and Storage

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for MO25 alpha recombinant protein

Recombinant full-length human MO25alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: MO25α (mouse protein 25 alpha) is a 40-kDa protein that interacts with the STE20-related adaptor-alpha (STRADα) pseudo kinase to form a regulatory complex capable of stimulating the activity of the LKB1 tumor suppressor protein kinase (1). LKB1 plays a critical role in cell proliferation, polarity and energy metabolism. LKB1 is mutated in the inherited Peutz-Jeghers cancer syndrome (PJS). MO25α binds directly to a conserved Trp-Glu-Phe sequence at the STRADα C terminus, and markedly enhances the binding of STRADα to LKB1 thereby increasing LKB1 catalytic activity (2).

Product Categories/Family for MO25 alpha recombinant protein

Cellular Proteins; Signaling Proteins - Cellular Proteins

References

1. Boudeau, J. et al: Analysis of the LKB1-STRAD-MO25 complex. J Cell Sci. 2004;117(Pt 26):6365-75.
2. Milburn, C.C. et al: Crystal structure of MO25 alpha in complex with the C terminus of the pseudo kinase STE20-related adaptor. Nat. Struct. Mol. Biol. 2004;11(2):193-200.

NCBI and Uniprot Product Information

NCBI GI #

195927018

NCBI GeneID

51719

NCBI Accession #

NP_057373.1

NCBI GenBank Nucleotide #

NM_016289

UniProt Accession #

Q9Y376

Molecular Weight

~63 kDa

NCBI Official Full Name

Homo sapiens calcium binding protein 39 (CAB39), transcript variant 1, mRNA

NCBI Official Synonym Full Names

calcium binding protein 39

NCBI Official Symbol

CAB39

NCBI Official Synonym Symbols

MO25; CGI-66

NCBI Protein Information

calcium-binding protein 39; MO25alpha

UniProt Protein Name

Calcium-binding protein 39

UniProt Gene Name

CAB39

UniProt Synonym Gene Names

MO25

UniProt Entry Name

CAB39_HUMAN

Uniprot Description

Function: Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1. Ref.6

Subunit structure: Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity. Ref.6

Subcellular location: Cytoplasm

Potential.

Sequence similarities: Belongs to the Mo25 family.

Research Articles on MO25 alpha

1. These data provide molecular understanding of the mechanism by which MO25 isoforms regulates the activity of STE20 family protein kinases.

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Product Notes

The MO25 alpha cab39 (Catalog #AAA515254) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full-length human MO25alpha was expressed by baculovirus in Sf9 insect cells. AAA Biotech's MO25 alpha can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the MO25 alpha cab39 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MO25 alpha, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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MO25 alpha recombinant protein