SETD6 recombinant protein

SETD6 Protein

Cat. Num. AAA515682

• Applications: Methyltransferase Assay
• Synonyms: SETD6; SETD6 Protein; SETD6 recombinant protein

• Host: Sf9 insect cells using baculovirus
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Positions: Full length
• Sequence Length: 2996

• Applicable Applications for SETD6 recombinant protein: Methyltransferase Assay
• Species: Human
• Tag: GST tag
• Preparation and Storage: Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Testing Data

Related Product Information for SETD6 recombinant protein

Description: Full-length recombinant human SETD6 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: SET domain-containing protein 6 (SETD6) is a mono-methyltransferase that has been shown to methylate RelA of NF-kappa-B complex and thus attenuate NF-kappa-B transcription factor activity. In addition, it monomethylates the histone H2A variant H2AZ which is required for the maintenance of embryonic stem cell self-renewal.

Product Categories/Family for SETD6 recombinant protein

Signaling Proteins; Acetyl/Methyltransferases

References

1. Levy D, et al: Lysine methylation of the NF-kappaB subunit RelA by SETD6 couples activity of the histone methyl-transferase GLP at chromatin to tonic repression of NF-kappaB signaling. Nat Immunol. 2011 12 (1):29-36. 2. Binda O, et al: SETD6 monomethylates H2AZ on lysine 7 and is required for the maintenance of embryonic stem cell self-renewal. Epigenetics. 2013 8 (2):177-83.

NCBI and Uniprot Product Information

• NCBI GI #: 971460852
• NCBI GeneID: 79918
• NCBI Accession #: NM_001160305.2
• NCBI GenBank Nucleotide #: NM_001160305
• Molecular Weight: ~75 kDa
• NCBI Official Full Name: Homo sapiens SET domain containing 6 (SETD6), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: SET domain containing 6
• NCBI Official Symbol: SETD6
• NCBI Protein Information: N-lysine methyltransferase SETD6
• UniProt Protein Name: N-lysine methyltransferase SETD6
• Protein Family: N-lysine methyltransferase
• UniProt Gene Name: SETD6

NCBI Description

This gene encodes a methyltransferase that adds a methyl group to the histone H2AZ, which is involved in nuclear receptor-dependent transcription. The protein also interacts with several endogenous proteins which are involved in nuclear hormone receptor signaling. A related pseudogene is located on chromosome 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

SETD6: Protein-lysine N-methyltransferase that specifically monomethylates 'Lys-310' of the RELA subunit of NF-kappa-B complex, leading to down-regulate NF-kappa-B transcription factor activity. Belongs to the SETD6 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.-; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function: NF-kappaB binding; protein binding; protein-lysine N-methyltransferase activity

Biological Process: inhibition of NF-kappaB transcription factor; peptidyl-lysine mono-methylation; regulation of inflammatory response; stem cell differentiation; stem cell maintenance