Rabbit MSK2 Polyclonal Antibody | anti-MSK2 antibody

MSK2 Antibody

Cat. Num. AAA9610944

• Gene Names: RPS6KA4; MSK2; RSK-B; S6K-alpha-4
• Reactivity: Human, Mouse; Predicted: Pig, Zebrafish, Bovine, Horse, Dog, Xenopus
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MSK2; Polyclonal Antibody; MSK2 Antibody; 90 kDa ribosomal protein S6 kinase 4; EC 2.7.11.1; KS6A4_HUMAN; Mitogen and stress activated protein kinase 2; Nuclear mitogen and stress activated protein kinase 2; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B; Ribosomal protein S6 kinase 90kD polypeptide 4; Ribosomal protein S6 kinase 90kDa polypeptide 4; Ribosomal protein S6 kinase alpha 4; Ribosomal protein S6 kinase alpha-4; RPS6KA4; RSKB; S6K-alpha-4; anti-MSK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Pig, Zebrafish, Bovine, Horse, Dog, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MSK2 Antibody detects endogenous levels of MSK2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 766

• Applicable Applications for anti-MSK2 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human MSK2.
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of MSK2 in lysates of HepG2, using MSK2 Antibody.)

Related Product Information for anti-MSK2 antibody

Description: Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors.

Post Translational Modifications: Ser-343 and Thr-568 phosphorylation is required for kinase activity. Ser-343 and Ser-196 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. Phosphorylated at Ser-343, Thr-568 and Thr-687 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha. Autophosphorylated at Ser-737 and Ser-745 by the N-terminal kinase domain (By similarity).

Subunit Structure: Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA4 associates with and phosphorylates the NF-kappa-B p65 subunit RELA.

Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 55743138
• NCBI GeneID: 8986
• NCBI Accession #: NP_001006945.1
• NCBI GenBank Nucleotide #: NP_001006945.1
• UniProt Accession #: O75676
• Molecular Weight: Observed: 85KD; Predicted: 86 kDa
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-4 isoform b
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase A4
• NCBI Official Symbol: RPS6KA4
• NCBI Official Synonym Symbols: MSK2; RSK-B; S6K-alpha-4
• NCBI Protein Information: ribosomal protein S6 kinase alpha-4
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-4
• UniProt Gene Name: RPS6KA4
• UniProt Synonym Gene Names: MSK2; S6K-alpha-4; RSKB
• UniProt Entry Name: KS6A4_HUMAN

NCBI Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Uniprot Description

MSK2: an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Phosphorylates H3 and HMG-14 in response to growth factors and cellular stress.

Protein type: EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; RSK family; MSK subfamily

Chromosomal Location of Human Ortholog: 11q11-q13

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; mitogen-activated protein kinase p38 binding; magnesium ion binding; ribosomal protein S6 kinase activity; ATP binding

Biological Process: axon guidance; regulation of transcription, DNA-dependent; positive regulation of histone phosphorylation; histone phosphorylation; negative regulation of cytokine production; activation of CREB transcription factor; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; protein amino acid phosphorylation; activation of NF-kappaB transcription factor