Typical Testing Data/Standard Curve (for reference only)
Interleukin-1alpha ELISA Kit | IL-1alpha elisa kit
Interleukin-1alpha (IL-1alpha)
Synonyms
Interleukin-1alpha; Interleukin-1alpha (IL-1alpha); Interleukin-1a (IL-1a); IL-1alpha elisa kit
Samples
Serum, Cell Culture Supernatant, and other biological fluids
Assay Type
Sandwich
Sensitivity
The minimum detectable dose of mouse IL-1alpha was determined by adding two standard deviations to the mean optical density value of 16 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of mouse IL-1alpha calculated from calibrate II diluted standard curve was <0.8pg/mL.
Intra-assay Precision
To determine within-run precision, three different samples of known concentration were assayed by using 16 replicates in 1 assay.
Inter-assay Precision
To determine between-run precision, three different samples of known concentration were measured by using replicates on 8 different assays with calibrator II as standard diluent.
Intended Uses
This Mouse IL-1alpha ELISA kit is to be used for the in vitro quantitative determination of mouse interleukin 1alpha (IL-1alpha) concentrations in serum, cell culture supernatant, and other biological fluids. This kit is intended for LABORATORY RESEARCH USE ONLY.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL-1alpha elisa kit
Principle of the assay: This mouse IL-1alpha enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse IL-1alpha. When standards or samples are added to the appropriate microtiter plate wells, mouse IL-1alpha in the standards or samples will be immobilized by the precoated antibody during incubation. Then, a biotin-conjugated antibody preparation specific for mouse IL-1alpha is added to each well and incubated. The biotin labelled antibody attaches to the wells by binding to mouse IL-1alpha. After plate washing, other proteins, components and unattached biotin labelled antibody is removed. After that, avidin-horseradish peroxidase (HRP) conjugate is added to each well. Avidin has a very high affinity for biotin, thus, it links the tracer (HRP) sturdily to the biotin labelled antibody. The wells are thoroughly washed to remove all unbound avidin-HRP conjugate and a TMB (3,3', 5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only wells that contain mouse IL-1alpha will exhibit a change in colour. The extent of colour change is proportional to the quantity of mouse IL-1alpha present in the standards/samples. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wave length of 450 nm +/- 2 nm. In order to measure the concentration of mouse IL-1alpha in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus IL-1alpha concentration (pg/mL). The concentration of mouse IL-1alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Background: IL-1alpha is a member of interleukin 1 family. IL-1alpha and IL-1beta recognize the same IL-1 receptor and share a number of similar biological functions. IL-1alpha is predominantly a cellassociated molecule whereas IL-1beta is a secreted molecule. IL-1alpha is synthesized primarily as a precursor that is biologically active via specific cell binding. Precursor IL-1alpha can be cleaved by extracellular proteases when the cells die, and can also be cleaved by activation of the calcium-dependent, membrane-associated calpains. Cleavage of the mouse IL-1alpha precursor results in an 18 kDa mature mouse IL-1alpha molecule. IL-1alpha is constitutively expressed by epithelial cells and the essential role of IL-1alpha in maintenance of skin barrier function. IL-1alpha can also produced by macrophages and neutrophils by the induction of microbes and microbial products. IL-1alpha can induce its own synthesis as well as the production of TNF and IL-6. IL-1alpha induces the production of IL-2, IL-2 receptors, GM-CSF and IL-4 from activated T cells, stimulates B cell proliferation and maturation, and increases immunoglobulin synthesis. IL-1alpha affects NK cell activation and LAK production associated with other cytokines, and induces prostaglandin synthesis in endothelial cells and smooth muscle cells, collagenase production in synovial cells, and cartilage and calcium resorption in bones. Studies have shown a connection between IL-1alpha and the pathogenesis of endometriotic lesions. The increased expression of both matrix-degrading MMP-1 and its major stimulatory cytokine IL-1alpha in endometriotic lesions and the selective co-expression in the stroma of endometriotic foci clearly suggests the involvement of the IL-1alpha molecule in the pathogenic mechanisms leading to local invasion and tissue destruction. Reports also indicate that the translation of the neurotransmitter gene only occurs after receiving IL-1alpha stimulation. This effect was suppressed by co-stimulation with IL-1 receptor antagonist. High levels of IL-1alpha are associated with sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, and atherosclerosis.
Background: IL-1alpha is a member of interleukin 1 family. IL-1alpha and IL-1beta recognize the same IL-1 receptor and share a number of similar biological functions. IL-1alpha is predominantly a cellassociated molecule whereas IL-1beta is a secreted molecule. IL-1alpha is synthesized primarily as a precursor that is biologically active via specific cell binding. Precursor IL-1alpha can be cleaved by extracellular proteases when the cells die, and can also be cleaved by activation of the calcium-dependent, membrane-associated calpains. Cleavage of the mouse IL-1alpha precursor results in an 18 kDa mature mouse IL-1alpha molecule. IL-1alpha is constitutively expressed by epithelial cells and the essential role of IL-1alpha in maintenance of skin barrier function. IL-1alpha can also produced by macrophages and neutrophils by the induction of microbes and microbial products. IL-1alpha can induce its own synthesis as well as the production of TNF and IL-6. IL-1alpha induces the production of IL-2, IL-2 receptors, GM-CSF and IL-4 from activated T cells, stimulates B cell proliferation and maturation, and increases immunoglobulin synthesis. IL-1alpha affects NK cell activation and LAK production associated with other cytokines, and induces prostaglandin synthesis in endothelial cells and smooth muscle cells, collagenase production in synovial cells, and cartilage and calcium resorption in bones. Studies have shown a connection between IL-1alpha and the pathogenesis of endometriotic lesions. The increased expression of both matrix-degrading MMP-1 and its major stimulatory cytokine IL-1alpha in endometriotic lesions and the selective co-expression in the stroma of endometriotic foci clearly suggests the involvement of the IL-1alpha molecule in the pathogenic mechanisms leading to local invasion and tissue destruction. Reports also indicate that the translation of the neurotransmitter gene only occurs after receiving IL-1alpha stimulation. This effect was suppressed by co-stimulation with IL-1 receptor antagonist. High levels of IL-1alpha are associated with sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, and atherosclerosis.
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Product Notes
The IL-1alpha (Catalog #AAA590058) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Interleukin-1alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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