Interleukin-2 ELISA Kit | IL-2 elisa kit

Interleukin-2 (IL-2)

Cat. Num. AAA590068

• Gene Names: IL2; IL-2; TCGF; lymphokine
• Synonyms: Interleukin-2; Interleukin-2 (IL-2); IL-2 elisa kit

• Sequence Length: 153

• Samples: Serum, Cell Culture Supernatant, and other biological fluids
• Assay Type: Sandwich
• Sensitivity: The minimum detectable dose of mouse IL-2 was determined by adding two standard deviations to the mean optical density value of 16 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of mouse IL-2 calculated from calibrate I diluted standard curve was <2.1pg/mL.
• Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by using 16 replicates in 1 assay.
• Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by using replicates on 8 different assays.
• Intended Uses: This Mouse IL-2 ELISA kit is to be used for the in vitro quantitative determination of mouse interleukin 2 (IL-2) concentrations in serum, cell culture supernatant, and other biological fluids. This kit is intended for LABORATORY RESEARCH USE ONLY.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-2 elisa kit

Principle of the assay: This mouse IL-2 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse IL-2. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse IL-2, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse IL-2 and other components in the samples. A biotin-conjugated antibody preparation specific for mouse IL-2 is added to each well and incubated. The biotin labelled antibody will attach to the wells by binding to mouse IL-2 present in the standards/samples. After plate washing, other proteins, components and unattached biotin labelled antibody are removed. Then, avidin-horseradish peroxidase (HRP) conjugate is added to each well. Avidin has a very high affinity for biotin, thus, it links the tracer (HRP) sturdily to the biotin conjugated antibody. The wells are thoroughly washed to remove all unbound avidin-HRP conjugate and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only the wells that contain mouse IL-2 will exhibit a change in colour. The extent of colour change is proportional to the quantity of mouse IL-2 present in the standards/samples. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of mouse IL-2 in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus IL-2 concentration (pg/mL). The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Background: Mouse IL-2 is a 17kDa protein produced by activated CD4+ T helper cells immediately after foreign antigens bind with the T-cell receptor. IL-2 is an initiator of the acquired immunity and a major clone expansion stimulator of activated T cells. The cytokine stimulates the growth, proliferation and differentiation of naïve T-cells into "effecter T-cells", promotes the survival of antigen specific "memory T-cell", and stimulates the maturation of "regulatory Tcells". The biological activity of IL-2 is mediated by receptor that is expressed primarily on the cell membrane of activated T-cells. IL-2 receptor is comprised of a specific alpha subunit with high affinity to IL-2, a beta subunit, and a common gamma subunit that is also involved with the biological functions of a number of other cytokines such as IL-4, IL-7, IL-9, IL-15 and IL-21. In addition to T cell clone expansion and haematopoietic effect, IL-2 also acts on activated B cells, monocytes, NK cells and LAK cells, and plays a role in tumor surveillance, antiinflammatory reaction and self antigen-tolerance. This ELISA kit provides a tool for studying IL-2 expression and regulation in animal model.

Product Categories/Family for IL-2 elisa kit

ELISA Kits for Mouse Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 28178861
• NCBI GeneID: 3558
• NCBI Accession #: NP_000577.2
• NCBI GenBank Nucleotide #: NM_000586.3
• Molecular Weight: 17,628 Da
• NCBI Official Full Name: interleukin-2
• NCBI Official Synonym Full Names: interleukin 2
• NCBI Official Symbol: IL2
• NCBI Official Synonym Symbols: IL-2; TCGF; lymphokine
• NCBI Protein Information: interleukin-2; T cell growth factor; aldesleukin; involved in regulation of T-cell clonal expansion
• UniProt Protein Name: Interleukin-2
• Protein Family: Interleukin
• UniProt Gene Name: IL2
• UniProt Entry Name: IL2_HUMAN

NCBI Description

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]

Uniprot Description

IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family.

Protein type: Cytokine; Secreted, signal peptide; Secreted; Oncoprotein

Chromosomal Location of Human Ortholog: 4q26-q27

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; interleukin-2 receptor binding; kinase activator activity; cytokine activity; carbohydrate binding; glycosphingolipid binding; kappa-type opioid receptor binding

Biological Process: positive regulation of isotype switching to IgG isotypes; negative regulation of heart contraction; natural killer cell activation; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; elevation of cytosolic calcium ion concentration; negative regulation of protein amino acid phosphorylation; cell-cell signaling; positive regulation of cell proliferation; protein kinase C activation; positive regulation of B cell proliferation; cell adhesion; T cell differentiation; positive regulation of interleukin-17 production; regulation of T cell homeostatic proliferation; positive regulation of regulatory T cell differentiation; positive regulation of immunoglobulin secretion; positive regulation of cell growth; positive regulation of interferon-gamma production; positive regulation of tissue remodeling; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; immune response; negative regulation of B cell apoptosis; negative regulation of apoptosis; positive regulation of inflammatory response