Monocyte Chemotactic Protein-3 ELISA Kit | MCP-3 elisa kit

Monocyte Chemotactic Protein-3 (MCP-3)

Cat. Num. AAA590022

• Synonyms: Monocyte Chemotactic Protein-3; Monocyte Chemotactic Protein-3 (MCP-3); MCP-3 elisa kit

• Specificity: This sandwich ELISA can recognises both natural and recombinant human MCP-3. This kit exhibits no significant cross-reactivity with human IL-8, IL-1beta, SAA, RANTES, MCAF, EGF, TNF-alpha, TGF-beta, M-CSF, GM-CSF, FGF, and EPO.
• Sequence Length: 109

• Samples: Serum, plasma, Cell Culture Supernatant, and other biological fluids
• Assay Type: Sandwich
• Sensitivity: The minimum detectable dose of MCP-3 was determined by adding two standard deviations to the mean optical density value of 20 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose using a standard curve generated with Calibrator Diluent I is 30 pg/mL and using Calibrator Diluent II is 20 pg/mL.
• Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by replicates of twenty in 1 assay.
• Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by replicates on 20 different assays.
• Intended Uses: This Human MCP-3 ELISA Kit is to be used for the in vitro quantitative determination of human monocyte chemotactic protein-3 (MCP-3) concentrations in serum, plasma, cell culture supernatant, and other biological fluids. This kit is intended for LABORATORY RESEARCH USE ONLY and is not for use in therapeutic procedures.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MCP-3 elisa kit

Principle of the assay: This MCP-3 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for MCP-3. Standards or samples are then added to the appropriate microtiter plate wells and incubated. MCP-3, if present, will bind and become immobilised by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound MCP-3 and other components of sample. In order to quantitate the amount of MCP-3 present in the sample, a standardised preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific for MCP-3 is added to each well to "sandwich" the MCP-3 immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MCP-3 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of MCP-3 in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus MCP-3 concentration (pg/mL). The concentration of MCP-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MCP-3 elisa kit

ELISA Kits for Human Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 313708
• UniProt Accession #: P80098; Q569J6
• Molecular Weight: 11,200 Da
• NCBI Official Full Name: monocyte chemotactic protein-3
• UniProt Protein Name: C-C motif chemokine 7
• UniProt Gene Name: CCL7
• UniProt Entry Name: CCL7_HUMAN

Uniprot Description

CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CCR1 chemokine receptor binding; chemokine activity; CCR2 chemokine receptor binding

Biological Process: cellular calcium ion homeostasis; monocyte chemotaxis; regulation of cell shape; cell-cell signaling; eosinophil chemotaxis; response to gamma radiation; immune response; cytoskeleton organization and biogenesis; signal transduction; inflammatory response; chemotaxis; positive regulation of cell migration