Interleukin-10 ELISA Kit | IL-10 elisa kit

Interleukin-10 (IL-10)

Cat. Num. AAA590015

• Gene Names: IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
• Synonyms: Interleukin-10; Interleukin-10 (IL-10); IL-10 elisa kit

• Specificity: This sandwich ELISA can detect both natural and recombinant human IL-10. This kit exhibits no significant cross-reactivity with human IL-1?, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNF-?, TNF-?, IFN-?. There is 2% reaction with recombinant mouse IL-10.
• Sequence Length: 178

• Samples: Cell Culture Supernatant, serum, plasma, and other biological fluids
• Assay Type: Sandwich
• Sensitivity: The minimum detectable quantities of human IL-10 as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 3.5 pg/mL and 3.7pg/mL respectively. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities.
• Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by replicates of twenty in 1 assay.
• Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by replicates on 25 different assays.
• Intended Uses: This Human Interleukin 10 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 10 (IL-10) concentrations in cell culture supernatant, serum, plasma, and other biological fluids. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not to be used in therapeutic procedures.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-10 elisa kit

Principle of the assay: This IL-10 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for IL-10 and incubated. IL-10, if present, will bind and become immobilized by the antibody pre-coated on the wells and then will become "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-10 and other components of the sample. In order to quantitatively determine the amount of IL-10 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of IL-10 within the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the standard provided is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-10 concentration (pg/mL). The concentration of IL-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL-10 elisa kit

ELISA Kits for Human Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 10835141
• NCBI GeneID: 3586
• NCBI Accession #: NP_000563.1
• NCBI GenBank Nucleotide #: NM_000572.2
• Molecular Weight: 20,517 Da
• NCBI Official Full Name: interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: IL10
• NCBI Official Synonym Symbols: CSIF; TGIF; GVHDS; IL-10; IL10A
• NCBI Protein Information: interleukin-10; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor
• UniProt Protein Name: Interleukin-10
• Protein Family: Interleukin
• UniProt Gene Name: IL10
• UniProt Entry Name: IL10_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]

Uniprot Description

IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; interleukin-10 receptor binding

Biological Process: negative regulation of chronic inflammatory response to antigenic stimulus; regulation of isotype switching; B cell proliferation; positive regulation of transcription, DNA-dependent; leukocyte chemotaxis; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; negative regulation of B cell proliferation; negative regulation of membrane protein ectodomain proteolysis; response to insulin stimulus; negative regulation of interferon-alpha biosynthetic process; cytoplasmic sequestering of NF-kappaB; negative regulation of T cell proliferation; cell-cell signaling; response to molecule of bacterial origin; negative regulation of interferon-gamma production; positive regulation of MHC class II biosynthetic process; negative regulation of interleukin-6 production; hemopoiesis; negative regulation of tumor necrosis factor biosynthetic process; inflammatory response; aging; response to drug; response to inactivity; negative regulation of interleukin-12 production; T-helper 2 type immune response; positive regulation of B cell apoptosis; negative regulation of nitric oxide biosynthetic process; negative regulation of myeloid dendritic cell activation; negative regulation of cytokine secretion during immune response; regulation of gene expression; B cell differentiation; defense response to bacterium; negative regulation of MHC class II biosynthetic process; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; response to activity; receptor biosynthetic process; regulation of sensory perception of pain; positive regulation of cytokine secretion; negative regulation of apoptosis

Disease: Graft-versus-host Disease, Susceptibility To; Rheumatoid Arthritis; Human Immunodeficiency Virus Type 1, Susceptibility To