Interleukin-15 ELISA Kit | IL-15 elisa kit

Interleukin-15 (IL-15)

Cat. Num. AAA590019

• Gene Names: IL15; IL-15
• Synonyms: Interleukin-15; Interleukin-15 (IL-15); IL-15 elisa kit

• Specificity: This sandwich ELISA can detect both natural and recombinant human IL-15. This kit exhibits no significant cross-reactivity with human IL-1, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNF-alpha, TNF-beta, TGF-beta1, TGF-beta2, G-CSF, GM-CSF.
• Sequence Length: 135

• Samples: Serum, plasma, and Cell Culture Supernatant
• Assay Type: Sandwich
• Sensitivity: The minimum detectable quantities of human IL-15 are 10 pg/mL and 9 pg/mL, when use a standard curve generated with Calibrator Diluent I and Calibrator Diluent II, respectively. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities
• Intended Uses: This Human Interleukin-15 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 15 (IL-15) concentrations in serum, plasma, and cell culture supernatant. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not for use in therapeutic procedures.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-15 elisa kit

Principle of the assay: This IL-15 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-15. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-15 and incubated. IL-15 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-15 and other components of the sample. In order to quantitatively determine the amount of IL-15 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethylbenzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-15, biotinconjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of IL-15 in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-15 concentration (pg/mL). The concentration of IL-15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL-15 elisa kit

ELISA Kits for Human Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 26787984
• NCBI GeneID: 3600
• NCBI Accession #: NP_751914.1
• NCBI GenBank Nucleotide #: NM_172174.2
• Molecular Weight: 14,912 Da
• NCBI Official Synonym Full Names: interleukin 15
• NCBI Official Symbol: IL15
• NCBI Official Synonym Symbols: IL-15
• NCBI Protein Information: interleukin-15
• UniProt Protein Name: Interleukin-15
• Protein Family: Interleukin
• UniProt Gene Name: IL15
• UniProt Entry Name: IL15_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported. [provided by RefSeq, Feb 2011]

Uniprot Description

IL15: Cytokine that stimulates the proliferation of T- lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted, signal peptide; Secreted; Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q31

Cellular Component: nucleoplasm; Golgi apparatus; extracellular space; cell surface; membrane; integral to plasma membrane; cytoplasm; extracellular region; endosome

Molecular Function: protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity

Biological Process: positive regulation of interleukin-17 production; positive regulation of natural killer cell proliferation; positive regulation of immune response; NK T cell proliferation; negative regulation of smooth muscle cell proliferation; cell maturation; signal transduction; lymph node development; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; extrathymic T cell selection; positive regulation of tissue remodeling; skeletal muscle atrophy; cell-cell signaling; natural killer cell differentiation; positive regulation of cell proliferation; positive regulation of T cell proliferation; immune response; regulation of T cell differentiation; regulation of defense response to virus by host; inflammatory response; hyaluronan metabolic process; aging; positive regulation of inflammatory response