Interleukin-1 beta ELISA Kit | IL-1beta elisa kit

Interleukin-1 beta (IL-1beta)

Cat. Num. AAA590006

• Gene Names: IL1B; IL1-BETA
• Synonyms: Interleukin-1 beta; Interleukin-1 beta (IL-1beta); Interleukin-1 beta (IL-1b); IL-1beta elisa kit

• Specificity: This sandwich ELISA can detect both natural and recombinant human IL-1beta. This kit exhibits no detectable cross-reactivity with human; IL-1alpha, IL-1RA, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IFN-alpha, IFN-gamma, TNF-alpha or mouse IL-1beta.
• Sequence Length: 268

• Samples: Cell Culture Supernatant, serum, plasma and other biological fluids
• Assay Type: Sandwich
• Sensitivity: The minimum detectable quantities of human IL-1beta as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 2.0 pg/mL and 2.0 pg/mL respectively. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities
• Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by replicates of 20 in 1 assay.
• Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by replicates on 20 different assays.
• Intended Uses: This Human IL-1beta ELISA kit is to be used for the in vitro quantitative determination of human interleukin-1beta (IL-1beta) concentrations in cell culture supernatant, serum, plasma and other biological fluids. This kit is intended for LABORATORY RESEARCH USE ONLY and is not for use in therapeutic procedures.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-1beta elisa kit

Principle of the assay: This IL-1beta enzyme linked immunosorbent assay (ELISA) applies a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-1beta. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for IL-1beta and incubated. IL-1beta, if present, will bind and become immobilized by the antibody pre-coated on the wells and then become "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-1beta and other components of the sample. In order to quantitatively determine the amount of IL-1beta present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-1beta, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of IL-1beta in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-1beta concentration (pg/mL). The concentration of IL-1beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL-1beta elisa kit

ELISA Kits for Human Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 126723555
• NCBI GeneID: 100008990
• NCBI Accession #: NP_001075670.1
• NCBI GenBank Nucleotide #: NM_001082201.1
• Molecular Weight: 30,665 Da
• NCBI Official Full Name: interleukin-1 beta
• NCBI Official Symbol: IL1B
• NCBI Official Synonym Symbols: IL1-BETA
• NCBI Protein Information: interleukin-1 beta; IL-1 beta; interleukin 1 beta; lymphocyte proliferation-potentiating factor
• UniProt Protein Name: Interleukin-1 beta
• Protein Family: Interleukin
• UniProt Gene Name: IL1B
• UniProt Entry Name: IL1B_RABIT

Uniprot Description

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.