Interleukin-16 ELISA Kit | IL-16 elisa kit

Interleukin-16 (IL-16)

Cat. Num. AAA590020

• Synonyms: Interleukin-16; Interleukin-16 (IL-16); IL-16 elisa kit

• Specificity: This ELISA recognizes both natural and recombinant human IL-16. This kit exhibits no detectable cross-reactivity with human; IL-1beta, IL-7, IL-8, IFN-gamma, EGF, EPO, GM-CSF, MCAF, MCP-3, M-CSF, SAA, TGF-beta, TNF-alpha.
• Sequence Length: 130

• Samples: Serum, plasma, Cell Culture Supernatant, and other biological fluids
• Assay Type: Sandwich
• Sensitivity: The minimum detectable dose of IL-16 was determined by adding two standard deviations to the mean optical density value of 20 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of human IL-16 using a standard curve generated with Calibrator Diluent I is 20 pg/mL and using Calibrator Diluent II is 18 pg/mL.
• Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by replicates of twenty in 1 assay.
• Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by replicates on 12 different assays.
• Intended Uses: This Human Interleukin-16 (IL-16) ELISA Kit is to be used for the in vitro quantitative determination of human IL-16 concentrations in serum, plasma, cell culture supernatant, and other biological fluids. This kit is intended FOR LABORATORY RESEARCH PURPOSES ONLY and is not for use in therapeutic procedures.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-16 elisa kit

Principle of the assay: This IL-16 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for IL-16. Standards or samples are then added to the appropriate microtiter plate wells and incubated. IL-16, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove any unbound IL-16 and other components of sample. In order to quantitatively determine the amount of IL-16 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific for IL-16 is added to each well to "sandwich" the IL-16 immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate solution are allowed to react over a short incubation period. Only those wells that contain IL-16 and enzyme-substrate reaction will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the colour change measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of IL-16 in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-16 (pg/mL). The concentration of IL-16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL-16 elisa kit

ELISA Kits for Human Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 2996198
• Molecular Weight: 59,345 Da
• NCBI Official Full Name: interleukin-16
• UniProt Protein Name: Pro-interleukin-16
• UniProt Gene Name: IL16
• UniProt Entry Name: IL16_HUMAN

Uniprot Description

IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Cytokine; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: extracellular space; cytoplasm; plasma membrane; nucleus

Molecular Function: cytokine activity

Biological Process: regulation of transcription, DNA-dependent; viral reproduction; transcription, DNA-dependent; leukocyte chemotaxis; immune response; induction of positive chemotaxis