Interleukin-4 ELISA Kit | IL-4 elisa kit

Interleukin-4 (IL-4)

Cat. Num. AAA590053

• Gene Names: IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1
• Synonyms: Interleukin-4; Interleukin-4 (IL-4); IL-4 elisa kit

• Sequence Length: 153

• Samples: Serum, Cell Culture Supernatant, and other biological fluids
• Assay Type: Sandwich
• Sensitivity: The minimum detectable dose of mouse IL-4 was determined by adding two standard deviations to the mean optical density value of 16 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of mouse IL-4 calculated from calibrate I diluted standard curve was <0.8pg/mL.
• Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by using 16 replicates in 1 assay.
• Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by using replicates on 8 different assays.
• Intended Uses: This Mouse IL-4 ELISA kit is to be used for the in vitro quantitative determination of mouse interleukin 4 (IL-4) concentrations in serum, cell culture supernatant, and other biological fluids. This kit is intended for LABORATORY RESEARCH USE ONLY.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-4 elisa kit

Principle of the assay: This mouse IL-4 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse IL-4. When standards or samples are added to the appropriate microtiter plate wells, mouse IL-4 in the standards or samples will be immobilized by the precoated antibody during incubation. Then, a biotin-conjugated antibody preparation specific for mouse IL-4 is added to each well and incubated. The biotin labelled antibody attaches to the wells by binding to mouse IL-4. After plate washing, other proteins, components and unattached biotin labelled antibody is removed. After that, avidin-horseradish peroxidase (HRP) conjugate is added to each well. Avidin has a very high affinity for biotin, thus, it links the tracer (HRP) sturdily to the biotin labelled antibody. The wells are thoroughly washed to remove all unbound avidin- HRP conjugate and a TMB (3,3', 5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only wells that contain mouse IL-4 will exhibit a change in colour. The extent of colour change is proportional to the quantity of mouse IL-4 present in the standards/samples. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wave length of 450 nm +/- 2 nm. In order to measure the concentration of mouse IL-4 in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus IL-4 concentration (pg/mL). The concentration of mouse IL- 4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Background: IL-4 is a very important B cell stimulation and differentiation factor. In addition, IL-4 modulates the immune function of other cell types including T cells, monocytes, macrophages, mast cells, fibroblasts, endothelial cells, osteoblasts, keratinocytes, hepatocytes, and astrocytes. IL-4 is mainly produced by CD4+ TH0 and TH2 cells, CD8+ T cells, and mast cells. IL-4 is the polarizing cytokine for TH2 phenotype. The gene for mouse IL-4 is mapped to chromosome 11 in close conjunction with genes for other T-helper 2 cytokines such as IL-13, GM-CSF, IL-5. Mouse IL-4 is a disulfide bond linked homodimer containing 113 amino acids in each subunit. The receptor for IL-4 is a heterodimer with a cytokine specific alpha unit, and a signal transduction beta subunit that is identical to the beta subunit of receptors for IL-13 and GM-CSF. IL-4 receptor alpha (IL- 4Ra) is expressed by eosinophils, B cells and basophils in member-bound form and soluble form. While the membrane bound form participates in the activation of eosinophils and B cells, the soluble form of IL-4Ra has an antagonist effect to IL-4. Increased IL-4 expression causes chronic IgE response and hypereosinophilic syndromes. Elevated IL-4 level is found in allergic diseases such as allergic rhinitis, asthma and allergic conjunctivitis.

Product Categories/Family for IL-4 elisa kit

ELISA Kits for Mouse Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4504669
• NCBI GeneID: 3565
• NCBI Accession #: NP_000580.1
• NCBI GenBank Nucleotide #: NM_000589.3
• Molecular Weight: 15,797 Da
• NCBI Official Full Name: interleukin-4 isoform 1
• NCBI Official Synonym Full Names: interleukin 4
• NCBI Official Symbol: IL4
• NCBI Official Synonym Symbols: BSF1; IL-4; BCGF1; BSF-1; BCGF-1
• NCBI Protein Information: interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; binetrakin; interleukin 4 variant 2; lymphocyte stimulatory factor 1; pitrakinra
• UniProt Protein Name: Interleukin-4
• Protein Family: Interleukin
• UniProt Gene Name: IL4
• UniProt Entry Name: IL4_HUMAN

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cell cycle regulation; Motility/polarity/chemotaxis; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; external side of plasma membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; interleukin-4 receptor binding

Biological Process: regulation of isotype switching; positive regulation of isotype switching to IgG isotypes; negative regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; microglial cell activation; regulation of proton transport; female pregnancy; positive regulation of activated T cell proliferation; chemotaxis; response to organic cyclic substance; positive regulation of interleukin-10 production; positive regulation of isotype switching to IgE isotypes; B cell costimulation; connective tissue growth factor biosynthetic process; positive regulation of MHC class II biosynthetic process; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; response to nutrient; response to drug; cholesterol metabolic process; regulation of immune response; negative regulation of chronic inflammatory response; T-helper 2 type immune response; regulation of phosphorylation; B cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; defense response to protozoan; T-helper 1 cell lineage commitment; positive regulation of chemokine biosynthetic process; response to ethanol; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of T cell differentiation; innate immune response in mucosa; retina development in camera-type eye; B cell differentiation; response to cytokine stimulus; cellular defense response; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; immune response; T-helper 2 cell differentiation; negative regulation of transcription, DNA-dependent; positive regulation of defense response to virus by host; negative regulation of apoptosis

Disease: Stroke, Ischemic