Typical Testing Data/Standard Curve (for reference only)
Mouse B7-1/CD80 ELISA Kit | B7-1/CD80 elisa kit
B7-1/CD80 (Mouse) ELISA
Reactivity
Mouse
Synonyms
B7-1/CD80; B7-1/CD80 (Mouse) ELISA; B7-1/CD80 elisa kit
Reactivity
Mouse
Samples
Tissue lysates or cell culture supernates
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
<10pg/ml
Intended Uses
For detection of CD80 in tissue lysates or cell culture supernates. The significance of detection in serum, plasma, body fluids is undetermined. For research use only.
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for B7-1/CD80 elisa kit
Principle of the assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CD80 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CD80 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CD80 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CD80 can be calculated.
Background: Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes. B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. The CD80 gene has 6 exons that span approximately 32 kb of genomic DNA. The antigen presentation coactivators B7-1 and B7-2 may be important for lymphocytic infiltration and the immune response against thyroid carcinoma.
Background: Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes. B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. The CD80 gene has 6 exons that span approximately 32 kb of genomic DNA. The antigen presentation coactivators B7-1 and B7-2 may be important for lymphocytic infiltration and the immune response against thyroid carcinoma.
Product Categories/Family for B7-1/CD80 elisa kit
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Product Notes
The Mouse B7-1/CD80 (Catalog #AAA494762) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494762 ELISA Kit recognizes Mouse B7-1/CD80. It is sometimes possible for the material contained within the vial of "B7-1/CD80, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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