Typical Testing Data/Standard Curve (for reference only)
Human Emmprin ELISA Kit | BSG elisa kit
Emmprin ELISA
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-E-Cadherin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-E-Cadherin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the E-Cadherin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of E-Cadherin can be calculated!!Background/Introduction: Cadherin-1 also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin is a protein that in humans is encoded by the CDH1 gene. E-cadherin is a Ca2+-dependent epithelial cell-cell adhesion molecule. Downregulation of Ecadherin expression often correlates with strong invasive potential and poor prognosis of Mouse carcinomas. It has 16 exons spanning approximately 100 kb of genomic DNA. The gene structure is similar to that of other cadherins. Ecadherin plays a central part in the process of epithelial morphogenesis. Expression of this protein is downregulated during the acquisition of metastatic potential at late stages of epithelial tumour progression.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
BSG: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Enriched on the surface of tumor cells. Up-regulated in gliomas. Its expression levels correlate with malignant potential of the tumor. Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms a complex with MMP1 at the tumor cell surface. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with ATP1B2, MAG and L1CAM. Interacts with AJAP1. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Immunoglobulin superfamily; Membrane protein, integral
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: Golgi membrane; focal adhesion; membrane; mitochondrion; integral to plasma membrane; plasma membrane; melanosome; sarcolemma; lipid raft; acrosomal membrane
Molecular Function: mannose binding; protein binding
Biological Process: response to peptide hormone stimulus; extracellular matrix organization and biogenesis; response to cAMP; decidualization; odontogenesis of dentine-containing teeth; response to mercury ion; extracellular matrix disassembly; cellular metabolic process; cell surface receptor linked signal transduction; pyruvate metabolic process; blood coagulation; leukocyte migration; embryo implantation
Disease: Blood Group--ok
Research Articles on BSG
Similar Products
Product Notes
The Human BSG bsg (Catalog #AAA494767) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494767 ELISA Kit recognizes Human BSG. It is sometimes possible for the material contained within the vial of "Emmprin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
