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Western Blot (WB) (LSP1 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the LSP1 antibody detected the LSP1 protein (arrow).)

Rabbit anti-Human LSP1 Polyclonal Antibody | anti-LSP1 antibody

LSP1, ID (LSP1, WP34, Lymphocyte-specific protein 1, 47kD actin-binding protein, 52kD phosphoprotein, Lymphocyte-specific antigen WP34) (PE)

Gene Names
LSP1; WP34; pp52
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
LSP1; Polyclonal Antibody; ID (LSP1; WP34; Lymphocyte-specific protein 1; 47kD actin-binding protein; 52kD phosphoprotein; Lymphocyte-specific antigen WP34) (PE); Lymphocyte-specific antigen WP34; anti-LSP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-LSP1 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
LSP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-144 amino acids from the Central region of human LSP1.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(LSP1 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the LSP1 antibody detected the LSP1 protein (arrow).)

Western Blot (WB) (LSP1 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the LSP1 antibody detected the LSP1 protein (arrow).)

Immunohistochemistry (IHC)

(LSP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LSP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (LSP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LSP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(LSP1 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (LSP1 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-LSP1 antibody
LSP1 is an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms.
Product Categories/Family for anti-LSP1 antibody
References
Davila, S., et al. Genes Immun. (2010). Odefrey, F., et al. Cancer Res. 70(4):1449-1458(2010). Bhatti, P., et al. Radiat. Res. 173(2):214-224(2010). Tamimi, R.M., et al. Cancer Causes Control (2010).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37kDa
NCBI Official Full Name
lymphocyte-specific protein 1 isoform 1
NCBI Official Synonym Full Names
lymphocyte specific protein 1
NCBI Official Symbol
LSP1
NCBI Official Synonym Symbols
WP34; pp52
NCBI Protein Information
lymphocyte-specific protein 1
UniProt Protein Name
Lymphocyte-specific protein 1
UniProt Gene Name
LSP1
UniProt Synonym Gene Names
WP34; pp52
UniProt Entry Name
LSP1_HUMAN

NCBI Description

This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

LSP1: May play a role in mediating neutrophil activation and chemotaxis. Binds actin. Activated T-lymphocytes.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: membrane; plasma membrane; actin cytoskeleton

Molecular Function: signal transducer activity; actin binding

Biological Process: cellular defense response; chemotaxis; cell motility; signal transduction

Research Articles on LSP1

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Product Notes

The LSP1 lsp1 (Catalog #AAA6317515) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LSP1, ID (LSP1, WP34, Lymphocyte-specific protein 1, 47kD actin-binding protein, 52kD phosphoprotein, Lymphocyte-specific antigen WP34) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LSP1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the LSP1 lsp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LSP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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