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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MAOB antibody.)

Rabbit anti-Human MAOB Polyclonal Antibody | anti-MAOB antibody

MAOB Antibody

Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were purified by affinity purification using immunogen.
Synonyms
MAOB; Polyclonal Antibody; MAOB Antibody; MGC26382; anti-MAOB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total MAOB protein.
Purity/Purification
Antibodies were purified by affinity purification using immunogen.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
520
Applicable Applications for anti-MAOB antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:100
Immunofluorescence: 1:50 - 1:200
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant protein of human MAOB.
Target Name
MAOB
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MAOB antibody.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MAOB antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using MAOB antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of U2OS cell using MAOB antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-MAOB antibody
The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position(PMID:3387449). This antibody is specific to MAOB.
Product Categories/Family for anti-MAOB antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,539 Da
NCBI Official Full Name
amine oxidase
NCBI Official Synonym Full Names
monoamine oxidase B
NCBI Official Symbol
MAOB
NCBI Protein Information
amine oxidase [flavin-containing] B
UniProt Protein Name
Amine oxidase [flavin-containing] B
Protein Family
UniProt Gene Name
MAOB
UniProt Synonym Gene Names
MAO-B
UniProt Entry Name
AOFB_HUMAN

NCBI Description

The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. [provided by RefSeq, Jul 2008]

Uniprot Description

MAOB: Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine. Belongs to the flavin monoamine oxidase family.

Protein type: Amino Acid Metabolism - histidine; Membrane protein, integral; Oxidoreductase; Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - arginine and proline; EC 1.4.3.4; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - phenylalanine; Mitochondrial; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: mitochondrial envelope; mitochondrial outer membrane; mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: amine oxidase activity; protein homodimerization activity; electron carrier activity; FAD binding

Biological Process: response to drug; negative regulation of serotonin secretion; positive regulation of dopamine metabolic process; response to selenium ion; substantia nigra development; response to ethanol; dopamine catabolic process; xenobiotic metabolic process; response to toxin; response to lipopolysaccharide; hydrogen peroxide biosynthetic process; response to corticosterone stimulus; response to aluminum ion

Research Articles on MAOB

Similar Products

Product Notes

The MAOB maob (Catalog #AAA9406806) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAOB Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAOB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:200. Researchers should empirically determine the suitability of the MAOB maob for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAOB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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