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Testing Data

Human alpha-Amylase Assay Kit

alpha-Amylase Inhibitor Screening Kit

Reactivity
Human
Synonyms
alpha-Amylase; alpha-Amylase Inhibitor Screening Kit; alpha amylase; alpha Amylase; alpha-amylase; alpha-Amylase assay kit
Ordering
For Research Use Only!
Reactivity
Human
Detection Method
Colorimetric: OD 405nm
Features & Benefits
Simple, 1-Step assay for screening/characterizing potential alpha-Amylase Inhibitors
Assay can be completed within one hour
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months

Testing Data

Testing Data
Related Product Information for alpha-Amylase assay kit
alpha-Amylases (EC 3.2.1.1) are digestive enzymes which catalyze the hydrolysis of internal alpha-1,4-glycosidic linkages in starch to lower molecular weight products, such glucose, maltose and maltotriose units. Human alpha-amylase is mainly expressed in salivary glands and pancreas. These two isozymes share a high degree of primary amino acid sequence similarity (97%) and 92% in their catalytic domains. Furthermore, both alpha-Amylases are immunologically identical in their reactions with polyclonal antibodies, share same mode of action, preferred substrates, are Chlorine activated and reach maximum activity at similar pH values. Functionally, they have similar but not identical cleavage patterns when tested with a variety of substrates. Modulation of alpha-amylase activity affects the utilization of carbohydrates as an energy source. This enzyme is responsible for the breakdown of complex carbohydrates in humans. Thus, inhibition of alpha-amylase could be considered as a strategy for the treatment of disorders related to carbohydrate uptake, such as diabetes, obesity, dental cavities and periodontal diseases. alpha-Amylase Inhibitor Screening Kit provides a simple, homogenous assay for screening potential alpha-Amylase inhibitors using a 96 well microplate. In our kit, human alpha-Amylase hydrolyzes the synthetic substrate, yielding smaller fragments containing the chromophore (pNP; OD = 405 nm). A potent, specific inhibitor is also included in the kit.

Similar Products

Product Notes

The Human alpha-Amylase (Catalog #AAA849699) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849699 Assay Kit recognizes Human alpha-Amylase. It is sometimes possible for the material contained within the vial of "alpha-Amylase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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