Typical Testing Data/Standard Curve (for reference only)
Cholesterol Assay Kit
Cholesterol Detection Kit (cell-based)
Synonyms
Cholesterol; Cholesterol Detection Kit (cell-based); Cholesterol assay kit
Application Notes
Screen/study/characterize stimulators/inhibitors that affect cholesterol transport and localization in a variety of cells.
Samples
Cells
Detection Method
Light and Fluorescence Microscopy (Ex/Em = 340-380/385-470 nm).
Features & Benefits
Simple, easy to perform, histochemical method of identification of unesterified cholesterol.
Includes U-18666A, a cholesterol transport inhibitor.
Includes U-18666A, a cholesterol transport inhibitor.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Cholesterol assay kit
Product Information: Cholesterol Detection Kit (cell-based): Simple histochemical method of identification of unesterified cholesterol in a variety of cells. 100 assays.
Background: Cholesterol is an essential structural component of animal cell membranes. It is required to maintain membrane structural integrity and fluidity. Cholesterol also serves as a precursor for the biosynthesis of several hormones, bile acid and vitamin D. Plasma membrane contains majority of the cellular cholesterol (80-90%) whereas little cholesterol resides in endoplasmic reticulum and in mitochondrial membrane. Transport of intracellular cholesterol within the cells to different compartments is through vesicular and non-vesicular pathways. Defects in these transport processes can alter cellular cholesterol metabolism resulting in pathological conditions. Filipin III is widely used as a probe for sterol localization in membranes. Interaction with cholesterol alters Filipin absorption and fluorescence spectra. This assay provides a simple, easy to perform, histochemical method of identification of unesterified cholesterol.
Summary: Detection method- Light and Fluorescence Microscopy (Ex/Em = 340-380/385-470 nm).
Applications- Screen/study/characterize stimulators/inhibitors that affect cholesterol transport and localization in a variety of cells.
Background: Cholesterol is an essential structural component of animal cell membranes. It is required to maintain membrane structural integrity and fluidity. Cholesterol also serves as a precursor for the biosynthesis of several hormones, bile acid and vitamin D. Plasma membrane contains majority of the cellular cholesterol (80-90%) whereas little cholesterol resides in endoplasmic reticulum and in mitochondrial membrane. Transport of intracellular cholesterol within the cells to different compartments is through vesicular and non-vesicular pathways. Defects in these transport processes can alter cellular cholesterol metabolism resulting in pathological conditions. Filipin III is widely used as a probe for sterol localization in membranes. Interaction with cholesterol alters Filipin absorption and fluorescence spectra. This assay provides a simple, easy to perform, histochemical method of identification of unesterified cholesterol.
Summary: Detection method- Light and Fluorescence Microscopy (Ex/Em = 340-380/385-470 nm).
Applications- Screen/study/characterize stimulators/inhibitors that affect cholesterol transport and localization in a variety of cells.
Product Categories/Family for Cholesterol assay kit
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Product Notes
The Cholesterol (Catalog #AAA841867) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Screen/study/characterize stimulators/inhibitors that affect cholesterol transport and localization in a variety of cells. Researchers should empirically determine the suitability of the Cholesterol for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cholesterol, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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