Human Fetuin A CLIA Kit | AHSG clia kit

Fetuin A (Human) LumiAb ELISA Kit

Cat. Num. AAA9510978

• Gene Names: AHSG; AHS; A2HS; HSGA; APMR1; FETUA
• Reactivity: Human
• Synonyms: Fetuin A; Fetuin A (Human) LumiAb ELISA Kit; Human Fetuin A; Alpha-2-HS-glycoprotein; Alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; AHSG clia kit

• Reactivity: Human
• Specificity: The LumiAb Human Fas Receptor ELISA is capable of recognizing both recombinant and naturally produced Human Fas Receptor proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 367

• Assay Type: Quantitative Sandwich
• Sensitivity: 15 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AHSG clia kit

Principle of the Assay: The LumiAb Human FasR Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human FasR concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human FasR while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human FASR is a novel class of transcriptional regulator that governs the activation of fatty acid biosynthesis genes. It acts as the receptor for TNFSF6/FASLG. In mechanism, the adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. In vitro, the secreted isoforms 2 to 6 block apoptosis. FASR contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins. Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A); also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.

Product Categories/Family for AHSG clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 112910
• NCBI GeneID: 197
• UniProt Accession #: P02765
• NCBI Official Full Name: Alpha-2-HS-glycoprotein
• NCBI Official Synonym Full Names: alpha 2-HS glycoprotein
• NCBI Official Symbol: AHSG
• NCBI Official Synonym Symbols: AHS; A2HS; HSGA; APMR1; FETUA
• NCBI Protein Information: alpha-2-HS-glycoprotein
• UniProt Protein Name: Alpha-2-HS-glycoprotein
• Protein Family: Alpha-2-HS-glycoprotein
• UniProt Gene Name: AHSG
• UniProt Synonym Gene Names: FETUA
• UniProt Entry Name: FETUA_HUMAN

NCBI Description

The protein encoded by this gene is a negatively-charged serum glycoprotein that is synthesized by hepatocytes. The encoded protein consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several processes, including endocytosis, brain development, and the formation of bone tissue. Defects in this gene are a cause of susceptibility to leanness. [provided by RefSeq, Aug 2017]

Uniprot Description

FETUA: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. Alpha-2-HS glycoprotein derives from this precursor, when the connecting peptide is cleaved off. The two chains A and B are held together by a single disulfide bond. Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. Belongs to the fetuin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space; extracellular region

Molecular Function: kinase inhibitor activity; cysteine protease inhibitor activity

Biological Process: negative regulation of bone mineralization; pinocytosis; ossification; positive regulation of phagocytosis; regulation of inflammatory response; acute-phase response; negative regulation of insulin receptor signaling pathway; negative regulation of phosphorylation; regulation of bone mineralization; skeletal development