ChimChagas2 Recombinant Protein

Recombinant chimeric antigen ChimChagas2 for Trypanosoma cruzi

Cat. Num. AAA505072

• Purity: >98% in a SDS-PAGE
• Synonyms: ChimChagas2; Recombinant chimeric antigen ChimChagas2 for Trypanosoma cruzi; Chimeric antigen; Tp44.5; Recombinant chimeric antigen for Chagas.; ChimChagas2 recombinant protein

• Host: Escherichia coli
• Specificity: The protein is immunoreactive against seropositive individuals to Chagas
• Purity/Purification: >98% in a SDS-PAGE
• Form/Format: Dry powder (lyophilized)
• Concentration: 1.21 mg/mL (varies by lot)

• Application Notes: ELISA assay. Where this product has not been tested for use in a particular technique, this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates. ; ; SUGGESTED TITER BY ELISA: approx. 1:48,000, which corresponds to 0.025 ug/ml of protein concentration in plates,for IgG detection.
• Composition: Components : his-ChimChagas2; Composition: Recombinant chimeric antigen with a his-tag in its N-terminus
• Buffer: 20 mM phosphate buffer pH 8.5 , 0.15 M NaCl, 5 mM EDTA and 0.1% polyoxyethylene(10) tridecyl ether
• QUALITY CONTROL: .1.- PROTEIN CONCENTRATION DETERMINED ESPECTROPHOTOMETRICALLY; ; DO₂₈₀= 0.147; A 0.1 %(=1 g/l)= 0.121 ; CONCENTRATION*: 0.121 mg/ml ; ; ; *The meassurement of the protein concentration has been performed; with the theoretical extinction coefficient of the recombinant ; protein obtained from Gill and vonHippel, 1989
• Molecular Weight Note: determined by SDS -PAGE, the protein band is between molecular markers of 66,200- 45,000 Da, while relative molecular mass calculated from amino acid sequence is 46,652.3 Da.
• ABSENCE OF PRECIPITATION AFTER A FREEZING AND THAWING CYCLE: ok
• Observations: In some cases, purified proteins run at a molecular weight which is slightly different to the theoretically calculated molecular weight maybe due to the his-tag presence or sequences of hydrophobic residues, which can produce a delay in SDS-PAGE. Proteins should be aintained frozen at high concentration. The dilution to be erformed for carrying out an LISA assays,should be freshly-made with a small quantity of protein. In order to defrost the protein, maintain the aliquot at 25ºC without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein,is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.
• Reconstitution: For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
• Preparation and Storage: Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.

Testing Data (TD)

(Figure 1. SDS-PAGE analysis (12.%) of 3 ul of recombinant ChimChagas 2. Purity is > 98% as determined by gel electrophoresis.Bands which are slightly smaller correspond to this same protein as it is showed in a western blot performed with a his-tag monoclonal antibody)

Testing Data (TD)

(The titer has been suggested in reference to an "in-house" ELISA kit performed .ELISA assays were performed with a serum sample panel of 25 positive-specimen sera and 5 negative specimen sera, validated by chimioluminiscence (Architect, Abbot)and obtained from the Andalusian Public health System Biobank.Each end user should carryout his own titration for his particular application.Figure 2. In this plot, the optical density at 450/620 nm for positive (blue) and negative (gray) IgG sera for Chagas disease are compared for each concentration of the recombinant antigen. An appropriate statistical test of significance for the comparison of means between both groups, the Welch's test, is employed. Eligible concentrations for the use of the antigen should present statistically significant differences between positive and negative sera. This happens when the intervals at the top do not overlap and, equivalently, when the p - value at the bottom is below 0.05. In the present figure, all p-values are below 0.05 and thus the intervals do not overlap. Therefore, any of the showed concentrations can be used to distinguish between positive and negative sera)

Testing Data (TD)

( This plot shows an indirect IgG ELISA assay performed with the ChimChagas2 recombinant antigen. the optical density at 450/620 nm for high-positive sera(dark blue), low-positive sera (light blue)and negative sera(gray) are displayed in the graphic)

Related Product Information for ChimChagas2 recombinant protein

The recombinant chimeric antigen for Chagas has been prepared as a chimeric protein formed by several antigenic regions from some antigens from this parasite.
Recombinant chimeric antigen for Chagas detection

Product Categories/Family for ChimChagas2 recombinant protein

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