Testing Data
Eukaryotes Soluble Epoxide Hydrolase Assay Kit
Soluble Epoxide Hydrolase Activity Assay Kit (Fluorometric)
Reactivity
Eukaryotes
Synonyms
Soluble Epoxide Hydrolase; Soluble Epoxide Hydrolase Activity Assay Kit (Fluorometric); Soluble Epoxide Hydrolase assay kit
Reactivity
Eukaryotes
Samples
Cell lysate(eg.HEK-293 or other cell types expected to have high sEHactivity)
Tissue lysate (eg. Liver tissue)
Recombinant enzyme
Purified protein
Tissue lysate (eg. Liver tissue)
Recombinant enzyme
Purified protein
Detection Method
Fluorescence: Ex/Em 362/460nm
Features & Benefits
Rapid, Simple & sensitive
Convenient & HTS
Includes specific sEH inhibitor
Rapid assessment of native/recombinant sEH activity.
Soluble Epoxide Hydrolase activity in mammalian cell culture, and tissue lysates
Recombinant enzyme, purified protein
Highly Sensitive Assay for the detection of Soluble Epoxide Hydrolase; Includes sEH Positive Control
Convenient & HTS
Includes specific sEH inhibitor
Rapid assessment of native/recombinant sEH activity.
Soluble Epoxide Hydrolase activity in mammalian cell culture, and tissue lysates
Recombinant enzyme, purified protein
Highly Sensitive Assay for the detection of Soluble Epoxide Hydrolase; Includes sEH Positive Control
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
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Testing Data
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Testing Data
Related Product Information for Soluble Epoxide Hydrolase assay kit
Soluble Epoxide Hydrolase or sEH (EC 3.3.2.10) is a cytosolic enzyme present ubiquitously in several organs including the liver, kidney, pancreatic islets, pituitary gland, lymphoid tissues, muscles, and the gastrointestinal tract. It catalyzes the hydrolysis of epoxyeicosatrienoic acids (EETs), i.e., epoxides derived from cytochrome P-450 mediated metabolism of arachidonic acid, and forming vicinal diols. sEH plays a major role in metabolism of endogenous lipids that are implicated in pain and inflammation. Soluble Epoxide Hydrolase Activity Assay Kit is a microplate based fluorometric kit for measuring sEH activity in cells and tissues as well as purified protein. It is based on the ability of sEH to hydrolyze a non-fluorescent substrate to a fluorescent product. The kit includes a specific inhibitor for soluble epoxide hydrolase, since the substrate can be hydrolyzed by non-specific hydrolases that are present in cell and tissue lysates. Specific sEH activity can be obtained by subtracting the activity in presence of sEH inhibitor form the total activitys.
Product Categories/Family for Soluble Epoxide Hydrolase assay kit
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Product Notes
The Eukaryotes Soluble Epoxide Hydrolase (Catalog #AAA849694) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849694 Assay Kit recognizes Eukaryotes Soluble Epoxide Hydrolase. It is sometimes possible for the material contained within the vial of "Soluble Epoxide Hydrolase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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