Human ZNRD1 (DNA-directed RNA polymerase I subunit RPA12) ELISA Kit | ZNRD1 elisa kit

Human ZNRD1 (DNA-directed RNA polymerase I subunit RPA12) ELISA Kit

Cat. Num. AAA7612246

• Gene Names: ZNRD1; TEX6; ZR14; HTEX6; Rpa12; hZR14; HTEX-6; TCTEX6; tctex-6
• Reactivity: Human
• Synonyms: ZNRD1 (DNA-directed RNA polymerase I subunit RPA12); Human ZNRD1 (DNA-directed RNA polymerase I subunit RPA12) ELISA Kit; ZNRD1; DNA-directed RNA polymerase I subunit RPA12; DNA-directed RNA polymerase I subunit H; Zinc ribbon domain-containing protein 1; POLR1H; RPA12; ZNRD1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ZNRD1. No significant cross-reactivity or interference between ZNRD1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ZNRD1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 525343323
• NCBI GeneID: 30834
• NCBI Accession #: NP_001265714.1
• NCBI GenBank Nucleotide #: NM_001278785.1
• UniProt Accession #: Q9P1U0
• Molecular Weight: 13,904 Da
• NCBI Official Full Name: DNA-directed RNA polymerase I subunit RPA12
• NCBI Official Synonym Full Names: zinc ribbon domain containing 1
• NCBI Official Symbol: ZNRD1
• NCBI Official Synonym Symbols: TEX6; ZR14; HTEX6; Rpa12; hZR14; HTEX-6; TCTEX6; tctex-6
• NCBI Protein Information: DNA-directed RNA polymerase I subunit RPA12; RNA polymerase I small specific subunit Rpa12; transcription-associated zinc ribbon protein
• UniProt Protein Name: DNA-directed RNA polymerase I subunit RPA12
• UniProt Gene Name: ZNRD1
• UniProt Synonym Gene Names: RPA12
• UniProt Entry Name: RPA12_HUMAN

NCBI Description

This gene encodes a DNA-directed RNA polymerase I subunit. The encoded protein contains two potential zinc-binding motifs and may play a role in regulation of cell proliferation. The encoded protein may be involved in cancer and human immunodeficiency virus progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

ZNRD1: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.

Protein type: Nucleolus; Transcription initiation complex; Transferase

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; DNA-directed RNA polymerase I complex

Molecular Function: nucleic acid binding; zinc ion binding

Biological Process: negative regulation of gene expression, epigenetic; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic