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Testing Data
Mammalian Chymotrypsin Assay Kit
Chymotrypsin Activity Assay Kit (Fluorometric)
Reactivity
Mammalian
Synonyms
Chymotrypsin; Chymotrypsin Activity Assay Kit (Fluorometric); Chymotrypsin assay kit
Reactivity
Mammalian
Samples
Cultured cell lysates(e.g. HeLa, MCF-7cells)
Tissue lysate(e.g. liver, pancreas, spleen)
Tissue lysate(e.g. liver, pancreas, spleen)
Detection Method
Fluorescence: Ex/Em 380/460nm
Features & Benefits
Simple procedure; takes ~ 1-2 hours
Fast and convenient
Measurement of chymotrypsin activity
Fast and convenient
Measurement of chymotrypsin activity
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
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Related Product Information for Chymotrypsin assay kit
Chymotrypsin (EC 3.4.21.1) is a key serine protease involved in dietary protein digestion in mammals. It is primarily produced by the pancreas, but may be expressed in other tissues, including spleen and liver. In the pancreas, chymotrypsin is initially expressed as the inactive proenzyme chymotrypsinogen, which is cleaved by other proteases to chymotrypsin, its active form. Chymotrypsin specifically cleaves peptide bonds at the C-terminal end of bulky hydrophobic or aromatic amino acids (such as tyrosine, tryptophan or phenylalanine). Chymotrypsin Activity Assay kit uses a synthetic fluorogenic substrate, enabling kinetic measurement of chymotrypsin activity in cell and tissue lysates. A chymotrypsin activator cleaves chymotrypsinogen to form active chymotrypsin, which then hydrolyzes the non-fluorescent substrate to release a stable fluorophore (Ex/Em = 380/460 nm). The kit includes a selective chymotrypsin inhibitor that can be used to measure specific chymotrypsin activity in samples containing non-specific proteases and endopeptidases that may also metabolize the substrate. The assay can detect as low as 0.01 mU of Chymotrypsin
Product Categories/Family for Chymotrypsin assay kit
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Product Notes
The Mammalian Chymotrypsin (Catalog #AAA849627) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849627 Assay Kit recognizes Mammalian Chymotrypsin. It is sometimes possible for the material contained within the vial of "Chymotrypsin, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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