Typical Testing Data/Standard Curve (for reference only)
Cathepsin B (CTSB) Assay Kit | CTSB assay kit
Cathepsin B (CTSB) Inhibitor Screening Kit (Fluorometric)
High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin B
Cathepsin B inhibitor, F-F-FMK is included in the kit
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Background: Cathepsin B (CTSB, EC 3.4.22.1) is a lysosomal cysteine proteinase that is suggested to participate in intracellular degradation and turnover of proteins. It has also been implicated in tumor invasion and metastasis. MyBioSource's Cathepsin B Inhibitor Screening Kit utilizes the ability of Cathepsin B to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin B-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize the potential inhibitors of Cathepsin B.
Summary: Detection method- Fluorescence (Ex/Em = 400/505 nm)
Applications- Screen/study/characterize potential inhibitors of Cathepsin B.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer disease, the most common cause of dementia. Overexpression of the encoded protein, which is a member of the peptidase C1 family, has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
CTSB: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Belongs to the peptidase C1 family.
Protein type: Motility/polarity/chemotaxis; Autophagy; EC 3.4.22.1; Protease
Chromosomal Location of Human Ortholog: 8p22
Cellular Component: extracellular space; mitochondrion; intracellular membrane-bound organelle; lysosome; perinuclear region of cytoplasm; melanosome; nucleolus; extracellular region; intracellular
Molecular Function: collagen binding; peptidase activity; proteoglycan binding; protein binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity
Biological Process: regulation of apoptosis; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; proteolysis involved in cellular protein catabolic process; epithelial cell differentiation; regulation of catalytic activity; toll-like receptor signaling pathway; innate immune response; decidualization; proteolysis
Research Articles on CTSB
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Product Notes
The CTSB ctsb (Catalog #AAA841714) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Screen/study/characterize potential inhibitors of Cathepsin B. Researchers should empirically determine the suitability of the CTSB ctsb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cathepsin B (CTSB), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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