Eogt recombinant protein

Eogt, 20-527aa, Mouse, His tag, Baculovirus

Cat. Num. AAA206347

• Gene Names: Eogt; Aer61; AI447490; AW214473; AW259391; A130022J15Rik
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: Eogt; 20-527aa; Mouse; His tag; Baculovirus; EGF domain-specific O-linked N-acetylglucosamine transferase; EOGT; A130022J15RiK; Aer61; AI447490; AW214473; AW259391; Eogt recombinant protein

• Host: Baculovirus
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: DKAHSEADDA PGKALYDYSS LRLPAEHIPF FLHNNRHVAS VCREDSHCPY KKHLENLNYC WGYEKSCAPE FRFGSPVCSY VDLGWTDTLE SAQDMFWRQA DFGYARERLG EIRTICQPER ASDSSLVCSR YLQYCRATGL YLDLRNIKRN HDRFKEDFLQ GGEIGGYCKL DSHALVSEGQ RKSPLQSWFA ELQGYTQLNF RPIEDAKCDI VVEKPTYFMK LDAGINMYHH FCDFLNLYLT QHVNNSFSTD VYIVMWDTST YGYGDLFSDT WKAFTDYDVI HLKTYDSKKV CFKEAVFSLL PRMRYGLFYN TPLISGCQNT GLFRAFSQHV LHRLNITQEG PKDGKVRVTI LARSTEYRKI LNQDELVNAL KTVSTFEVRV VDYKYRELGF LDQLRITHNT DIFIGMHGAG LTHLLFLPDW AAVFELYNCE DERCYLDLAR LRGIHYITWR KPSKVFPQDK GHHPTLGEHP KFTNYSFDVE EFMYLVLQAA EHVLQHPQWP FKKKHDELLE HHHHHH
• Sequence Length: 527

• Applicable Applications for Eogt recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Eogt recombinant protein

EOGT, also known as EGF domain-specific O-linked N-acetylglucosamine transferase, is involved in the regulation of Notch receptor. O-GlcNAc (O-linked b-N-acetylglucosamine) is introduced by a single intracellular O-GlcNAc transferase (OGT) and a single extracellular O-GlcNAc transferase (EOGT). O-GlcNAc results from the addition of a single N-acetylglucosamine residue to serine/threonine residues. Recombinant mouse EOGT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Eogt recombinant protein

Enzymes & Proteases; Other

References

Hart GW., et al. (2007) Nature 446:1017-1022.; Sakaidani Y., et al. (2012) Biochem Biophys Res Commun. 419:14-19.

NCBI and Uniprot Product Information

• NCBI GI #: 30424992
• NCBI GeneID: 101351
• NCBI Accession #: NP_780522
• NCBI GenBank Nucleotide #: NM_175313.4
• UniProt Accession #: Q8BYW9
• Molecular Weight: 60.4kDa (516aa) 50-70KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: EGF domain-specific O-linked N-acetylglucosamine transferase
• NCBI Official Synonym Full Names: EGF domain-specific O-linked N-acetylglucosamine (GlcNAc) transferase
• NCBI Official Symbol: Eogt
• NCBI Official Synonym Symbols: Aer61; AI447490; AW214473; AW259391; A130022J15Rik
• NCBI Protein Information: EGF domain-specific O-linked N-acetylglucosamine transferase
• UniProt Protein Name: EGF domain-specific O-linked N-acetylglucosamine transferase
• Protein Family: EGF domain-specific O-linked N-acetylglucosamine transferase
• UniProt Gene Name: Eogt
• UniProt Synonym Gene Names: Aer61; Eogt1

Uniprot Description

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains.