SDS-Page
RNASE3 recombinant protein
RNASE3, 28-160aa, Human,His-tag, Baculovirus
Gene Names
RNASE3; ECP; RAF1; RNS3
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
RNASE3; 28-160aa; Human; His-tag; Baculovirus; Ribonuclease; RNase A family; 3 (eosinophil cationic protein); ECP; RAF1; RNS3; RNASE3 recombinant protein
Host
Baculovirus
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence
ADPRPPQFTR AQWFAIQHIS LNPPRCTIAM RAINNYRWRC KNQNTFLRTT FANVVNVCGN QSIRCPHNRT LNNCHRSRFR VPLLHCDLIN PGAQNISNCR YADRPGRRFY VVACDNRDPR DSPRYPVVPV HLDTTIHHHH HH
Sequence Length
160
Applicable Applications for RNASE3 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for RNASE3 recombinant protein
RNASE3, also known as Ribonuclease RNase A family 3, located in the eosinophil primary matrix. It released during degranulation of eosinophils. This protein is related to inflammation and asthma because in these cases, there are increased levels of ECP in the body. It is a potent cytotoxic protein capable of killing cells of guinea pig tracheal epithelium, mammalian leukemia, epidermis carcinoma, and breast carcinoma, as well as non-mammalian cells such as parasites, bacteria, and viruses. Recombinant human RNASE3 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for RNASE3 recombinant protein
References
Kang I. et al., (2010) Eur Arch Otorhinolaryngol.267:391-395. ; Rubin J. et al., (2013) Mol Immunol. 55:372-380.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16.6kDa (142aa) 18-28KDa (SDS-PAGE under reducing conditions.)
NCBI Official Full Name
Ribonuclease, RNase A family, 3 (eosinophil cationic protein)
NCBI Official Synonym Full Names
ribonuclease A family member 3
NCBI Official Symbol
RNASE3
NCBI Official Synonym Symbols
ECP; RAF1; RNS3
NCBI Protein Information
eosinophil cationic protein
UniProt Protein Name
Eosinophil cationic protein
Protein Family
UniProt Gene Name
RNASE3
UniProt Synonym Gene Names
ECP; RNS3; ECP; RNase 3
NCBI Description
The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]
Uniprot Description
Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
Research Articles on RNASE3
Similar Products
Product Notes
The RNASE3 rnase3 (Catalog #AAA206514) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's RNASE3 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the RNASE3 rnase3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ADPRPPQFTR AQWFAIQHIS LNPPRCTIAM RAINNYRWRC KNQNTFLRTT FANVVNVCGN QSIRCPHNRT LNNCHRSRFR VPLLHCDLIN PGAQNISNCR YADRPGRRFY VVACDNRDPR DSPRYPVVPV HLDTTIHHHH HH. It is sometimes possible for the material contained within the vial of "RNASE3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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