KLK2 recombinant protein

KLK2, 25-261aa, Human, His-tag, Baculovirus

Cat. Num. AAA206405

• Gene Names: KLK2; hK2; hGK-1; KLK2A2
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: KLK2; 25-261aa; Human; His-tag; Baculovirus; Kallikerin-2 isoform1; hGK-1; hK2; KLK2A2; Glandular kallikrein 2 Glandular kallikrein-1; hGK 1; Kallikrein 2 prostatic; Kallikrein related peptidase 2; kallikrein; glandular; Kallikrein; prostatic; Kallikrein-2; KLK 2 Klk-2; Klk2; KLK2_HUMAN; KLK2A 2; KLK2A2 MGC12201; Tissue kallikrein 2; Tissue kallikrein-2 Ton; KLK2 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPIVGGWEC EKHSQPWQVA VYSHGWAHCG GVLVHPQWVL TAAHCLKKNS QVWLGRHNLF EPEDTGQRVP VSHSFPHPLY NMSLLKHQSL RPDEDSSHDL MLLRLSEPAK ITDVVKVLGL PTQEPALGTT CYASGWGSIE PEEFLRPRSL QCVSLHLLSN DMCARAYSEK VTEFMLCAGL WTGGKDTCGG DSGGPLVCNG VLQGITSWGP EPCALPEKPA VYTKVVHYRK WIKDTIAANP HHHHHH
• Sequence Length: 261

• Applicable Applications for KLK2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for KLK2 recombinant protein

KLK2, also known as kallikerin-2 isoform1, is a secreted serine protease that is highly expressed in the human prostate gland. The enzyme is highly specific for cleavage after arginine residues. This protein is able to activate the urokinase-type plasminogen activator. It is inhibited by serpins such as protein C inhibitor, antichymotrypsin and plasminogen activator inhibitor. Recombinant human KLK2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for KLK2 recombinant protein

Signal Transduction; Enzymes & Proteases

References

Frenette G., et al. (1997) Int J Cancer. 71:897-899.; Mikolajczyk SD., et al. (1999) Cancer Res. 59:3927-3930.;

NCBI and Uniprot Product Information

• NCBI GI #: 5031829
• NCBI GeneID: 3817
• NCBI Accession #: NP_005542
• NCBI GenBank Nucleotide #: NM_005551.4
• UniProt Accession #: P20151; Q15946; Q9UJZ9; B4DU93; B4DUB0; F5H8L3
• Molecular Weight: 27.2kDa (246aa) 28-40KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: kallikrein-2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: kallikrein related peptidase 2
• NCBI Official Symbol: KLK2
• NCBI Official Synonym Symbols: hK2; hGK-1; KLK2A2
• NCBI Protein Information: kallikrein-2
• UniProt Protein Name: Kallikrein-2
• Protein Family: Kallikrein
• UniProt Gene Name: KLK2
• UniProt Synonym Gene Names: hGK-1

NCBI Description

This gene encodes a member of the grandular kallikrein protein family. Kallikreins are a subgroup of serine proteases that are clustered on chromosome 19. Members of this family are involved in a diverse array of biological functions. The protein encoded by this gene is a highly active trypsin-like serine protease that selectively cleaves at arginine residues. This protein is primarily expressed in prostatic tissue and is responsible for cleaving pro-prostate-specific antigen into its enzymatically active form. This gene is highly expressed in prostate tumor cells and may be a prognostic maker for prostate cancer risk. Alternate splicing results in both coding and non-coding transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.