Porcine Prostaglandin E Synthase 2 ELISA Kit | PTGES2 elisa kit

Porcine Prostaglandin E Synthase 2 ELISA Kit

Cat. Num. AAA737418

• Gene Names: PTGES2; GBF1; GBF-1; PGES2; C9orf15; mPGES-2
• Reactivity: Porcine
• Synonyms: Prostaglandin E Synthase 2; Porcine Prostaglandin E Synthase 2 ELISA Kit; PTGES2 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This PTGES2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine PTGES2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTGES2 elisa kit

Principle of the assay: PTGES2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTGES2 antibody and an PTGES2-HRP conjugate. The assay sample and buffer are incubated together with PTGES2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTGES2 concentration since PTGES2 from samples and PTGES2-HRP conjugate compete for the anti-PTGES2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PTGES2 from the sample, fewer sites are left to bind PTGES2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTGES2 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13376617
• NCBI GeneID: 80142
• NCBI Accession #: NP_079348.1
• NCBI GenBank Nucleotide #: NM_025072.6
• Molecular Weight: 41,943 Da
• NCBI Official Full Name: prostaglandin E synthase 2 isoform 1
• NCBI Official Synonym Full Names: prostaglandin E synthase 2
• NCBI Official Symbol: PTGES2
• NCBI Official Synonym Symbols: GBF1; GBF-1; PGES2; C9orf15; mPGES-2
• NCBI Protein Information: prostaglandin E synthase 2; GATE-binding factor 1; microsomal prostaglandin E synthase-2; membrane-associated prostaglandin E synthase 2; gamma-interferon-activated transcriptional element-binding factor 1
• UniProt Protein Name: Prostaglandin E synthase 2
• Protein Family: Prostaglandin E synthase
• UniProt Gene Name: PTGES2
• UniProt Synonym Gene Names: C9orf15; PGES2; mPGES-2
• UniProt Entry Name: PGES2_HUMAN

NCBI Description

The protein encoded by this gene is a membrane-associated prostaglandin E synthase, which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. This protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE). Multiple transcript variants have been found for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

PTGES2: Isomerase that catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction with CEBPB; however, the relevance of transcription activation activity remains unclear. Belongs to the GST superfamily.

Protein type: Isomerase; EC 5.3.99.3; Membrane protein, integral; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: Golgi membrane; mitochondrion; perinuclear region of cytoplasm; integral to membrane; nucleus; cytosol

Molecular Function: electron carrier activity; glutathione binding; DNA binding; lyase activity; protein disulfide oxidoreductase activity; heme binding; prostaglandin-E synthase activity

Biological Process: secretion; cell redox homeostasis; positive regulation of transcription, DNA-dependent; prostaglandin biosynthetic process