Testing Data
Poly(A) Polymerase Enzyme
Poly(A) Polymerase, Yeast
Applications
Labelling of RNA with ATP or cordycepinPoly(A) tailing of RNA for cloning or affinity purification
Increasing translation of RNA transferred into eukaryotic cells
Synonyms
Poly(A) Polymerase; Yeast; Poly(A) Polymerase enzyme
Applicable Applications for Poly(A) Polymerase enzyme
Labelling of RNA with ATP or cordycepin
Poly(A) tailing of RNA for cloning or affinity purification
Increasing translation of RNA transferred into eukaryotic cells
Poly(A) tailing of RNA for cloning or affinity purification
Increasing translation of RNA transferred into eukaryotic cells
Storage Buffer
20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% (v/v) Glycerol.
Notes
One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37 degree C.
Preparation and Storage
Store all components at -20 degree C.
Testing Data
Testing Data
Related Product Information for Poly(A) Polymerase enzyme
Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3' ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single dA residue to the 3'-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E Coli.
Similar Products
Product Notes
The Poly(A) Polymerase (Catalog #AAA4156430) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Poly(A) Polymerase can be used in a range of immunoassay formats including, but not limited to, Labelling of RNA with ATP or cordycepin Poly(A) tailing of RNA for cloning or affinity purification Increasing translation of RNA transferred into eukaryotic cells. Researchers should empirically determine the suitability of the Poly(A) Polymerase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Poly(A) Polymerase, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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