Testing Data
T4 RNA Ligase 2 Enzyme
T4 RNA Ligase 2 (dsRNA Ligase)
Applications
Ligating nicks in double-stranded RNALigating the 3'OH of RNA to the 5'phosphate of DNA
Synonyms
T4 RNA Ligase 2; T4 RNA Ligase 2 (dsRNA Ligase); T4 RNA Ligase 2 enzyme
Applicable Applications for T4 RNA Ligase 2 enzyme
Ligating nicks in double-stranded RNA
Ligating the 3'OH of RNA to the 5'phosphate of DNA
Ligating the 3'OH of RNA to the 5'phosphate of DNA
Storage Buffer
10 mM Tris-HCl (pH 7.5), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 35 mM (NH4) 2SO4 and 50% (v/v) Glycerol.
Notes
One unit is defined as the amount of T4 RNA Ligase 2 that ligates 0.4 ug of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction volume of 20 ul in 30 minutes at 37 degree C.
Preparation and Storage
Store all components at -20 degree C.
Testing Data
Testing Data
Related Product Information for T4 RNA Ligase 2 enzyme
T4 RNA Ligase 2 (dsRNA Ligase) catalyzes the ATP-dependent formation of a 3'†'5' phosphodiester bond catalyzing intramolecular and intermolecular RNA strand joining. Unlike T4 RNA Ligase 1 (Cat. No. E010) which ligates single-stranded RNA, T4 RNA Ligase 2 joins knicks on double-stranded RNA and can also ligate the 3'OH of RNA to the 5' phosphate of DNA in a double stranded structure. T4 RNA Ligase 2 requires ATP for activity unless the substrate is preadenylated on the 5' end in which case a truncated version of T4 RNA Ligase 2 (Cat. No. E012) is a better enzyme because it generates less side-reaction ligation products than the full length enzyme.
Similar Products
Product Notes
The T4 RNA Ligase 2 (Catalog #AAA4156424) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's T4 RNA Ligase 2 can be used in a range of immunoassay formats including, but not limited to, Ligating nicks in double-stranded RNA Ligating the 3'OH of RNA to the 5'phosphate of DNA. Researchers should empirically determine the suitability of the T4 RNA Ligase 2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "T4 RNA Ligase 2, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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