Testing Data
T4 Polynucleotide Enzyme
T4 Polynucleotide Kinase
Applications
Labelling 5'-termini of DNA or RNA to be used as: primers for DNA sequencingo primers for PCR, probes for hybridization, probes for transcript mapping, markers for gel electrophoresisAddition of 5'-phosphates to oligonucleotides, PCR products, and DNA
Synonyms
T4 Polynucleotide; T4 Polynucleotide Kinase; T4 Polynucleotide enzyme
Applicable Applications for T4 Polynucleotide enzyme
Labelling 5'-termini of DNA or RNA to be used as: primers for DNA sequencingo primers for PCR, probes for hybridization, probes for transcript mapping, markers for gel electrophoresis
Addition of 5'-phosphates to oligonucleotides, PCR products, and DNA
Addition of 5'-phosphates to oligonucleotides, PCR products, and DNA
Storage Buffer
10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 uM ATP, and 50% (v/v) Glycerol.
Notes
One unit is defined as the amount of T4 Polynucleotide Kinase that catalyzes the incorporation of 1 nmol gamma-phosphate from ATP to the 5'-hydroxyl termini of micrococcal nuclease-treated DNA in 30 minutes at 37 degree C in 1X T4 Polynucleotide Kinase Reaction Buffer.
Preparation and Storage
Store all components at -20 degree C.
Testing Data
Testing Data
Related Product Information for T4 Polynucleotide enzyme
T4 Polynucleotide Kinase catalyzes the transfer of the gamma-phosphate from ATP to the 5'-hydroxyl terminus of double and single-stranded RNA and DNA, oligonucleotides or nucleoside 3'-monophosphates. The enzyme is also capable of catalyzing the removal of 3'-phosphoryl groups from 3'-phosphoryl polynucleotides, deoxynucleoside 3'-monophosphates and deoxynucleoside 3'-diphosphates.
Similar Products
Product Notes
The T4 Polynucleotide (Catalog #AAA4156419) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's T4 Polynucleotide can be used in a range of immunoassay formats including, but not limited to, Labelling 5'-termini of DNA or RNA to be used as: primers for DNA sequencingo primers for PCR, probes for hybridization, probes for transcript mapping, markers for gel electrophoresis Addition of 5'-phosphates to oligonucleotides, PCR products, and DNA. Researchers should empirically determine the suitability of the T4 Polynucleotide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "T4 Polynucleotide, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
