Testing Data
T4 RNA Ligase 1 Enzyme | rnlA enzyme
T4 RNA Ligase 1 (ssRNA Ligase)
Gene Names
rnlA; 63
Applications
RNA 3'-termini labeling with cytidine 3', 5'-bis [alpha-32P] phosphateIntermolecular and intramolecular joining of RNA and DNA
Incorporation of unnatural amino acids into proteins
Synthesis of oligoribonucleotides and oligodeoxyribonucleotides
Synonyms
T4 RNA Ligase 1; T4 RNA Ligase 1 (ssRNA Ligase); rnlA enzyme
Sequence Length
374
Applicable Applications for rnlA enzyme
RNA 3'-termini labeling with cytidine 3', 5'-bis [alpha-32P] phosphate
Intermolecular and intramolecular joining of RNA and DNA
Incorporation of unnatural amino acids into proteins
Synthesis of oligoribonucleotides and oligodeoxyribonucleotides
Intermolecular and intramolecular joining of RNA and DNA
Incorporation of unnatural amino acids into proteins
Synthesis of oligoribonucleotides and oligodeoxyribonucleotides
Storage Buffer
10 mM Tris-HCl (pH 7.5), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT and 50% (v/v) Glycerol.
Notes
One unit is defined as the amount of T4 RNA Ligase 1 that converts 1 nmole of 5'-[32P]rA16 into a phosphatase-resistant form in 30 minutes at 37 degree C.
Preparation and Storage
Store all components at -20 degree C.
Testing Data
Testing Data
Related Product Information for rnlA enzyme
T4 RNA Ligase 1 catalyzes the ATP-dependent formation of a 3'†'5' phosphodiester bond on a broad range of substrates including single-stranded RNA and DNA, oligoribonucleotides, oligodeoxynucleotides, as well as numerous nucleotide derivatives. The phosphodiester bond is created between a 5-phosphoryl-terminated nucleic acid donor and a 3-hydroxyl-terminated nucleic acid acceptor in a template-independent manner.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
43,509 Da
NCBI Official Full Name
T4 RNA ligase 1
NCBI Official Symbol
rnlA
NCBI Official Synonym Symbols
63
NCBI Protein Information
tail fiber attachment and RNA ligase for repair of tRNA in restrictive prr hosts, in conjunction with PseT
UniProt Protein Name
T4 RNA ligase 1
Protein Family
UniProt Gene Name
63
UniProt Synonym Gene Names
gp63
Uniprot Description
Involved in countering a host defense mechanism which activates T4-induced anticodon nuclease and shuts off viral translation. Repairs 5'-PO4 and 3'-OH groups in tRNA(Lys).
Research Articles on rnlA
Similar Products
Product Notes
The rnlA 63 (Catalog #AAA4156423) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's T4 RNA Ligase 1 can be used in a range of immunoassay formats including, but not limited to, RNA 3'-termini labeling with cytidine 3', 5'-bis [alpha-32P] phosphate Intermolecular and intramolecular joining of RNA and DNA Incorporation of unnatural amino acids into proteins Synthesis of oligoribonucleotides and oligodeoxyribonucleotidesDisclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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