Testing Data
T4 DNA Polymerase Enzyme
T4 DNA Polymerase
Applications
Generates blunt end DNA by filling in 5'-overhangs or/and removing 3'-overhangsHigh fidelity due to strong 3'†'5' exonuclease activity
Synthesis of labeled DNA probes by the replacement reaction
Site-specific mutagenesis via primer extension fro
Synonyms
T4 DNA Polymerase; T4 DNA Polymerase enzyme
Applicable Applications for T4 DNA Polymerase enzyme
Generates blunt end DNA by filling in 5'-overhangs or/and removing 3'-overhangs
High fidelity due to strong 3'†'5' exonuclease activity
Synthesis of labeled DNA probes by the replacement reaction
Site-specific mutagenesis via primer extension fro
High fidelity due to strong 3'†'5' exonuclease activity
Synthesis of labeled DNA probes by the replacement reaction
Site-specific mutagenesis via primer extension fro
Storage Buffer
100 mM KPO4 (pH 6.5), 1 mM DTT, and 50% (v/v) Glycerol.
Notes
One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37 degree C using poly(dA-dT) :poly(dA-dT) as a template:primer.
Preparation and Storage
Store all components at -20 degree C.
Testing Data
Testing Data
Related Product Information for T4 DNA Polymerase enzyme
T4 DNA Polymerase catalyzes the 5'†'3' synthesis of DNA from a single stranded, primed DNA template. This high fidelity enzyme also has potent 3'†'5' exonuclease activity but lacks 5'†'3' exonuclease function. T4 DNA Polymerase is the ideal choice for creating blunt ended DNA by removing 3'-overhangs or 5'-overhang filling, and is also useful for second strand DNA synthesis in site-specific mutagenesis.
Similar Products
Product Notes
The T4 DNA Polymerase (Catalog #AAA4156418) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's T4 DNA Polymerase can be used in a range of immunoassay formats including, but not limited to, Generates blunt end DNA by filling in 5'-overhangs or/and removing 3'-overhangs High fidelity due to strong 3'†'5' exonuclease activity Synthesis of labeled DNA probes by the replacement reaction Site-specific mutagenesis via primer extension fro. Researchers should empirically determine the suitability of the T4 DNA Polymerase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "T4 DNA Polymerase, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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