T7 RNA Polymerase Enzyme | T7p07 enzyme

T7 RNA Polymerase

Cat. Num. AAA4156442

• Applications: Synthesis of RNA transcripts for hybridization probes; Synthesis of RNA for in vitro translation; Synthesis of biologically active mRNA; Generate large amounts of labelled or non-labelled RNA
• Synonyms: T7 RNA Polymerase; T7p07 enzyme

• Sequence Length: 883

• Applicable Applications for T7p07 enzyme: Synthesis of RNA transcripts for hybridization probes; Synthesis of RNA for in vitro translation; Synthesis of biologically active mRNA; Generate large amounts of labelled or non-labelled RNA
• Storage Buffer: 50 mM Tris-HCl (pH 8), 1 mM EDTA, 20 mM beta-ME, 100 mM NaCl, 0.1% TritonX-100 and 50% (v/v) Glycerol.
• Notes: One unit is defined as the amount of T7 RNA Polymerase that is required to incorporate 1 nmol ATP into acid-insoluble material in a 50 ul reaction volume in 1 hour at 37 degree C in 1X T7 RNA Polymerase Reaction Buffer.
• Preparation and Storage: Store all components at -20 degree C.

Testing Data

Related Product Information for T7p07 enzyme

T7 RNA Polymerase is a DNA dependent RNA polymerase that catalyzes the synthesis of RNA in the 5'†' 3' direction only in the presence of its cognate T7 phage promoter sequence. T7 RNA Polymerase has high specificity for the T7 phage promoter and will not recognize SP6 or T3 RNA Polymerase promoter sequences.

NCBI and Uniprot Product Information

• NCBI GI #: 12643995
• NCBI GeneID: 1261050
• UniProt Accession #: P00573; Q38543
• Molecular Weight: 98,855 Da
• NCBI Official Full Name: T7 RNA polymerase
• NCBI Official Symbol: T7p07
• NCBI Protein Information: T3/T7-like RNA polymerase
• UniProt Protein Name: T7 RNA polymerase
• Protein Family: T7 RNA polymerase
• UniProt Gene Name: 1

Uniprot Description

Highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. Recognizes a specific promoter sequence and enters first into an "abortive phase" where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. Unwinds the double-stranded DNA to expose the coding strand for templating. Participates in the initiation of viral DNA replication presumably by making primers accessible to the DNA polymerase, thus facilitating the DNA opening. Plays also a role in viral DNA packaging, probably by pausing the transcription at the right end of concatemer junction to allow packaging complex recruitment and beginning of the packaging process.