Testing Data
Klenow Fragment (3'-->5' Exo-) Enzyme
Klenow Fragment (3'-->5' Exo-)
Applications
Dideoxy DNA sequencing of single-or double-stranded DNA templatescDNA second-strand synthesis
Generate single-stranded DNA probes using random primers
Site-directed DNA mutagenesis using synthetic oligonucleotides
Synonyms
Klenow Fragment (3'-->5' Exo-); Klenow Fragment (3'-->5' Exo-) enzyme
Applicable Applications for Klenow Fragment (3'-->5' Exo-) enzyme
Dideoxy DNA sequencing of single-or double-stranded DNA templates
cDNA second-strand synthesis
Generate single-stranded DNA probes using random primers
Site-directed DNA mutagenesis using synthetic oligonucleotides
cDNA second-strand synthesis
Generate single-stranded DNA probes using random primers
Site-directed DNA mutagenesis using synthetic oligonucleotides
Storage Buffer
25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.
Notes
One unit is defined as the amount of Klenow Fragment (3'†'5' Exo-) that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37 degree C using poly(dA-dT) :poly(dA-dT) as a template:primer.
Preparation and Storage
Store all components at -20 degree C.
Testing Data
Testing Data
Related Product Information for Klenow Fragment (3'-->5' Exo-) enzyme
Klenow Fragment (3'-->5' Exo-) is the large fragment of E Coli DNA Polymerase I which retains polymerase activity but lacks 5'†'3' exonuclease activity and has mutations that effectively abolish the inherent 3'†'5' exonuclease activity.
Similar Products
Product Notes
The Klenow Fragment (3'-->5' Exo-) (Catalog #AAA4156439) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Klenow Fragment (3'-->5' Exo-) can be used in a range of immunoassay formats including, but not limited to, Dideoxy DNA sequencing of single-or double-stranded DNA templates cDNA second-strand synthesis Generate single-stranded DNA probes using random primers Site-directed DNA mutagenesis using synthetic oligonucleotides. Researchers should empirically determine the suitability of the Klenow Fragment (3'-->5' Exo-) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Klenow Fragment (3'-->5' Exo-), Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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